Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides

There is abundant evidence that reactive oxygen species are implicated in several physiological and pathological processes. To protect biological targets from oxidative damage. antioxidants must react with radicals and other reactive species faster than biological substrates do. The aim of the prese...

Descripción completa

Detalles Bibliográficos
Autores: Khalil, Najeh M. [UNESP], Pepato, Maria Teresa [UNESP], Brunetti, Iguatemy Lourenço [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/7354
Acceso en línea:http://dx.doi.org/10.4067/S0716-97602008000200006
http://hdl.handle.net/11449/7354
Access Level:acceso abierto
Palabra clave:ABTS decolorization assay
antioxidant activity
superoxide anion scavenging activity
id BR_ba5ff0e0932fa80f968b6dbb6a08c8ee
oai_identifier_str oai:repositorio.unesp.br:11449/7354
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoidesABTS decolorization assayantioxidant activitysuperoxide anion scavenging activityThere is abundant evidence that reactive oxygen species are implicated in several physiological and pathological processes. To protect biological targets from oxidative damage. antioxidants must react with radicals and other reactive species faster than biological substrates do. The aim of the present study was to determine the in vitro antioxidant activity of aqueous extracts from leaves of Bauhinia forficata Link (Fabaceae - Caesalpinioideae) and Cissus sicyoides L. (Vitaceae) (two medicinal plants used popularly in the control of diabetes mellitus), using several different assay systems, namely, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) decolorization. superoxide anion radical (O-2 center dot-) scavenging and myeloperoxidase (MPO) activity. In the ABTS assay for total antioxidant activity, B. forficata showed IC50 8.00 +/- 0.07 mu g/mL, while C. sicyoides showed IC50 13.0 +/- 0.2 mu g/mL. However, the extract of C. sicyoides had a stronger effect on O-2 center dot- (IC50 60.0 +/- 2.3 mu p/mL) than the extract of B. forficata (IC50 90.0 +/- 4.4 mu g/mL). B. forficata also had a stronger inhibitory effect on MPO activity, as measured by guaiacol oxidation, than C. sicyoides. These results indicate that aqueous extracts of leaves of B. forficata and C. sicyoides are a potential source of natural antioxidants and may be helpful in the prevention of diabetic complications associated with oxidative stress.São Paulo State Univ, UNESP, Sch Pharmaceut Sci, Clin Anal Dept, São Paulo, BrazilSão Paulo State Univ, UNESP, Sch Pharmaceut Sci, Clin Anal Dept, São Paulo, BrazilSoc Biolgia ChileUniversidade Estadual Paulista (Unesp)2014-05-20T13:24:00Z2014-05-20T13:24:00Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article165-171application/pdfhttp://dx.doi.org/10.4067/S0716-97602008000200006Biological Research. Santiago: Soc Biolgia Chile, v. 41, n. 2, p. 165-171, 2008.0716-9760http://hdl.handle.net/11449/7354S0716-97602008000200006WOS:000259578800006S0716-97602008000200006-en.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Research2.3570,654info:eu-repo/semantics/openAccessKhalil, Najeh M. [UNESP]Pepato, Maria Teresa [UNESP]Brunetti, Iguatemy Lourenço [UNESP]2025-03-29T05:26:45Zoai:repositorio.unesp.br:11449/7354Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-03-29T05:26:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
title Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
spellingShingle Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
Khalil, Najeh M. [UNESP]
ABTS decolorization assay
antioxidant activity
superoxide anion scavenging activity
title_short Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
title_full Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
title_fullStr Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
title_full_unstemmed Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
title_sort Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
dc.creator.none.fl_str_mv Khalil, Najeh M. [UNESP]
Pepato, Maria Teresa [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
author Khalil, Najeh M. [UNESP]
author_facet Khalil, Najeh M. [UNESP]
Pepato, Maria Teresa [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
author_role author
author2 Pepato, Maria Teresa [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv ABTS decolorization assay
antioxidant activity
superoxide anion scavenging activity
topic ABTS decolorization assay
antioxidant activity
superoxide anion scavenging activity
description There is abundant evidence that reactive oxygen species are implicated in several physiological and pathological processes. To protect biological targets from oxidative damage. antioxidants must react with radicals and other reactive species faster than biological substrates do. The aim of the present study was to determine the in vitro antioxidant activity of aqueous extracts from leaves of Bauhinia forficata Link (Fabaceae - Caesalpinioideae) and Cissus sicyoides L. (Vitaceae) (two medicinal plants used popularly in the control of diabetes mellitus), using several different assay systems, namely, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) decolorization. superoxide anion radical (O-2 center dot-) scavenging and myeloperoxidase (MPO) activity. In the ABTS assay for total antioxidant activity, B. forficata showed IC50 8.00 +/- 0.07 mu g/mL, while C. sicyoides showed IC50 13.0 +/- 0.2 mu g/mL. However, the extract of C. sicyoides had a stronger effect on O-2 center dot- (IC50 60.0 +/- 2.3 mu p/mL) than the extract of B. forficata (IC50 90.0 +/- 4.4 mu g/mL). B. forficata also had a stronger inhibitory effect on MPO activity, as measured by guaiacol oxidation, than C. sicyoides. These results indicate that aqueous extracts of leaves of B. forficata and C. sicyoides are a potential source of natural antioxidants and may be helpful in the prevention of diabetic complications associated with oxidative stress.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2014-05-20T13:24:00Z
2014-05-20T13:24:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0716-97602008000200006
Biological Research. Santiago: Soc Biolgia Chile, v. 41, n. 2, p. 165-171, 2008.
0716-9760
http://hdl.handle.net/11449/7354
S0716-97602008000200006
WOS:000259578800006
S0716-97602008000200006-en.pdf
url http://dx.doi.org/10.4067/S0716-97602008000200006
http://hdl.handle.net/11449/7354
identifier_str_mv Biological Research. Santiago: Soc Biolgia Chile, v. 41, n. 2, p. 165-171, 2008.
0716-9760
S0716-97602008000200006
WOS:000259578800006
S0716-97602008000200006-en.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biological Research
2.357
0,654
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 165-171
application/pdf
dc.publisher.none.fl_str_mv Soc Biolgia Chile
publisher.none.fl_str_mv Soc Biolgia Chile
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1853671471314894848
score 15,301603

Similares en LA Referencia