Effects of aerobic exercise on cardiac function and gene expression of NADPH oxidases in diaphragm muscle of rats with aortic stenosis-induced heart failure

We evaluated the influence of aerobic physical exercise (EX) on gene-encoding proteins associated with oxidative stress in diaphragm muscle of rats with aortic stenosis-induced heart failure (HF). Wistar male rats were divided into four groups: Control sedentary (C); Control exercise (C-Ex); Sedenta...

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Detalhes bibliográficos
Autores: Janini Gomes, Mariana, Sabela, Ana Karenina, Ferreira, Isabele Tiburcio Pecin, de Souza, Sérgio Luiz Borges [UNESP], Mota, Gustavo Augusto Ferreira [UNESP], da Silva, Vitor Loureiro [UNESP], de Campos, Dijon Henrique Salomé [UNESP], Lima, Aline Regina Ruiz [UNESP], Carvalho, Marianna Rabelo, Bazan, Silmeia Garcia Zanati [UNESP], Corrêa, Camila Renata [UNESP], Cicogna, Antônio Carlos [UNESP], Okoshi, Marina Politi [UNESP], Pacagnelli, Francis Lopes
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/303312
Acesso em linha:http://dx.doi.org/10.3389/fphys.2023.1182303
https://hdl.handle.net/11449/303312
Access Level:acceso abierto
Palavra-chave:aerobic physical exercise
aortic stenosis
diaphragm
heart failure
oxidative stress
Descrição
Resumo:We evaluated the influence of aerobic physical exercise (EX) on gene-encoding proteins associated with oxidative stress in diaphragm muscle of rats with aortic stenosis-induced heart failure (HF). Wistar male rats were divided into four groups: Control sedentary (C); Control exercise (C-Ex); Sedentary aortic stenosis (AS); Aortic stenosis exercise (AS-Ex). Exercised rats trained 5 times a week for 10 weeks on a treadmill. Statistical analysis was performed by ANOVA or Kruskal–Wallis test. In the final echocardiogram, animals with aortic stenosis subjected to exercise demonstrated improvement in systolic function compared to the sedentary aortic stenosis group. In diaphragm muscle, the activity of antioxidant enzymes, malondialdehyde malondialdehyde concentration, protein carbonylation, and protein expression of p65 and its inhibitor IκB did not differ between groups. Alterations in gene expression of sources that generate reactive species of oxygen were observed in AS-Ex group, which showed decreased mRNA abundance of NOX2 and NOX4 compared to the aortic stenosis group (p < 0.05). We concluded that aerobic exercise has a positive impact during heart failure, ameliorating systolic dysfunction and biomarkers of oxidative stress in diaphragm muscle of rats with aortic stenosis-induced heart failure.

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