Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds

In response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, re...

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Autores: Campos, Thiago Souza [UNESP], Pereira, Vania M., El Merzougui, Soumaya, Beleski, David, Pérez, Héctor E., Pivetta, Kathia Fernandes Lopes [UNESP], Vendrame, Wagner A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/303591
Acceso en línea:http://dx.doi.org/10.3390/horticulturae10121256
https://hdl.handle.net/11449/303591
Access Level:acceso abierto
Palabra clave:bignoniaceae
germination
plant species conservation
radiographic imaging
seed quality
vitrification solutions
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spelling Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seedsbignoniaceaegerminationplant species conservationradiographic imagingseed qualityvitrification solutionsIn response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, reduced to 6.5% through desiccation. X-ray imaging allowed for detailed visualization of internal structures, identifying seeds as normal, abnormal, or dead based on damage and development. Normal seeds consistently germinated and produced healthy seedlings, while those with internal damage or excessive desiccation either resulted in abnormal seedlings or did not germinate. Various cryopreservation treatments were tested, including storage at −80 °C and liquid nitrogen immersion (LN), with and without vitrification solutions (PSV2; PVS3; PSV2 + 1% phloroglucinol; PSV3 + 1% phloroglucinol). Results indicated that immersion in LN without cryoprotectants achieved the highest germination and seedling viability, whereas vitrification solutions, such as PVS2 and PVS3, negatively affected germination. This study demonstrates that X-ray imaging is an effective tool for assessing seed quality and detecting internal damage, while cryopreservation without cryoprotectants is suitable for long-term seed storage. This work highlights the benefits of combining X-ray assessment with optimized cryopreservation techniques to support the conservation of threatened species.Agricultural Sciences Department Agricultural and Veterinarian Sciences Faculty São Paulo State University, SPEnvironmental Horticulture Department Institute of Food and Agricultural Sciences University of FloridaLaboratory of Biotechnology and Valorization of Natural Resources Sciences Faculty Ibn Zohr UniversityAgricultural Sciences Department Agricultural and Veterinarian Sciences Faculty São Paulo State University, SPUniversidade Estadual Paulista (UNESP)University of FloridaIbn Zohr University2025-04-29T19:30:06Z2024-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/horticulturae10121256Horticulturae, v. 10, n. 12, 2024.2311-7524https://hdl.handle.net/11449/30359110.3390/horticulturae101212562-s2.0-85213356720Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHorticulturaeinfo:eu-repo/semantics/openAccessCampos, Thiago Souza [UNESP]Pereira, Vania M.El Merzougui, SoumayaBeleski, DavidPérez, Héctor E.Pivetta, Kathia Fernandes Lopes [UNESP]Vendrame, Wagner A.2025-10-22T05:19:08Zoai:repositorio.unesp.br:11449/303591Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T05:19:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
title Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
spellingShingle Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
Campos, Thiago Souza [UNESP]
bignoniaceae
germination
plant species conservation
radiographic imaging
seed quality
vitrification solutions
title_short Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
title_full Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
title_fullStr Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
title_full_unstemmed Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
title_sort Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
dc.creator.none.fl_str_mv Campos, Thiago Souza [UNESP]
Pereira, Vania M.
El Merzougui, Soumaya
Beleski, David
Pérez, Héctor E.
Pivetta, Kathia Fernandes Lopes [UNESP]
Vendrame, Wagner A.
author Campos, Thiago Souza [UNESP]
author_facet Campos, Thiago Souza [UNESP]
Pereira, Vania M.
El Merzougui, Soumaya
Beleski, David
Pérez, Héctor E.
Pivetta, Kathia Fernandes Lopes [UNESP]
Vendrame, Wagner A.
author_role author
author2 Pereira, Vania M.
El Merzougui, Soumaya
Beleski, David
Pérez, Héctor E.
Pivetta, Kathia Fernandes Lopes [UNESP]
Vendrame, Wagner A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Florida
Ibn Zohr University
dc.subject.por.fl_str_mv bignoniaceae
germination
plant species conservation
radiographic imaging
seed quality
vitrification solutions
topic bignoniaceae
germination
plant species conservation
radiographic imaging
seed quality
vitrification solutions
description In response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, reduced to 6.5% through desiccation. X-ray imaging allowed for detailed visualization of internal structures, identifying seeds as normal, abnormal, or dead based on damage and development. Normal seeds consistently germinated and produced healthy seedlings, while those with internal damage or excessive desiccation either resulted in abnormal seedlings or did not germinate. Various cryopreservation treatments were tested, including storage at −80 °C and liquid nitrogen immersion (LN), with and without vitrification solutions (PSV2; PVS3; PSV2 + 1% phloroglucinol; PSV3 + 1% phloroglucinol). Results indicated that immersion in LN without cryoprotectants achieved the highest germination and seedling viability, whereas vitrification solutions, such as PVS2 and PVS3, negatively affected germination. This study demonstrates that X-ray imaging is an effective tool for assessing seed quality and detecting internal damage, while cryopreservation without cryoprotectants is suitable for long-term seed storage. This work highlights the benefits of combining X-ray assessment with optimized cryopreservation techniques to support the conservation of threatened species.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-01
2025-04-29T19:30:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/horticulturae10121256
Horticulturae, v. 10, n. 12, 2024.
2311-7524
https://hdl.handle.net/11449/303591
10.3390/horticulturae10121256
2-s2.0-85213356720
url http://dx.doi.org/10.3390/horticulturae10121256
https://hdl.handle.net/11449/303591
identifier_str_mv Horticulturae, v. 10, n. 12, 2024.
2311-7524
10.3390/horticulturae10121256
2-s2.0-85213356720
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Horticulturae
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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