COMPARISON AMONG DECONTAMINATION METHODS AT INITIAL PHASE OF BANANA IN VITRO CULTURE
Four decontamination methods for in vitro culture of banana (Musa AAB cv. Maçã) explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from axillary buds were submersed in different concentrations of NaOCl for each different period of t...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Brasil |
| Institución: | Universidade Federal de Goiás (UFG) |
| Repositorio: | Pesquisa Agropecuária Tropical (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.revistas.ufg.br:article/2856 |
| Acceso en línea: | https://revistas.ufg.br/pat/article/view/2856 |
| Access Level: | acceso abierto |
| Palabra clave: | Banana cv. Maçã tissue culture superficial sterilization Musa AAB cultura de tecidos esterilização superficial |
| Sumario: | Four decontamination methods for in vitro culture of banana (Musa AAB cv. Maçã) explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from axillary buds were submersed in different concentrations of NaOCl for each different period of time. After being reduced to 8mm x 5mm x 5mm, explants were transferred to modified MS media, which were kept in the dark for 10 days, followed by growth chamber at 28°C +/- 2°C with 16h light. Best results were obtained with 40mm x 20mm x 20mm or 40mm x 15mm x 15mm explants, immersed once or twice in 3.5% NaOCl. Higher contamination levels were observed 15 days after the beginning of the experiment; at 1% NaOCl, however, 96.67% contamination was observed after 3 days. Bacterial incidence was higher than fungal. KEY-WORDS: Banana cv. Maçã; tissue culture; superficial sterilization; Musa AAB. |
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