Intrathecal Transplantation of Autologous and Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Dogs

The route used in the transplantation of mesenchymal stem cells (MSCs) can directly affect the treatment success. The transplantation of MSCs via the intrathecal (IT) route can be an important therapeutic strategy for neurological disorders. The objective of this study was to evaluate the safety and...

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Detalles Bibliográficos
Autores: Benavides, Felipe Pérez [UNESP], Pinto, Giovana Boff Araujo [UNESP], Heckler, Marta Cristina Thomas [UNESP], Hurtado, Diana Milena Rodríguez [UNESP], Teixeira, Livia Ramos [UNESP], Monobe, Marina Mitie de Souza [UNESP], Machado, Gisele Fabrino [UNESP], de Melo, Guilherme Dias [UNESP], Rodríguez-Sánchez, Diego Noé [UNESP], Alvarenga, Fernanda da Cruz Landim e [UNESP], Amorim, Rogério Martins [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/229488
Acceso en línea:http://dx.doi.org/10.1177/09636897211034464
http://hdl.handle.net/11449/229488
Access Level:acceso abierto
Palabra clave:bone marrow mesenchymal stem cells
cell-based therapy
cerebrospinal fluid
intrathecal route
matrix metalloproteinase
Descripción
Sumario:The route used in the transplantation of mesenchymal stem cells (MSCs) can directly affect the treatment success. The transplantation of MSCs via the intrathecal (IT) route can be an important therapeutic strategy for neurological disorders. The objective of this study was to evaluate the safety and feasibility of the IT transplantation of autologous (Auto-MSCs) and allogeneic (Allo-MSCs) bone marrow mesenchymal stem cells (BM-MSCs) in healthy dogs. Based on neurodisability score, cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI), no significant differences from the control group were observed on day 1 or day 5 after IT Auto- or Allo-MSCs transplantation (P > 0.05). In addition, analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression in the CSF revealed no significant differences (P > 0.05) at 5 days after IT transplantation in the Auto- or Allo-MSCs group when compared to the control. Intrathecal transplantation of BM-MSCs in dogs provides a safe, easy and minimally invasive route for the use of cell-based therapeutics in central nervous system diseases.

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