Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)

This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 μM) and...

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Detalles Bibliográficos
Autores: Castro, Ana Hortência, Braga, Karina, Sousa, Fernanda, Coimbra, Mairon, Chagas, Rafael César
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Revista ciência agronômica (Online)
Idioma:inglés
OAI Identifier:oai:periodicos.ufc:article/84604
Acceso en línea:http://periodicos.ufc.br/revistacienciaagronomica/article/view/84604
Access Level:acceso abierto
Palabra clave:Cerrado
Medicinal plant
In vitro culture
Secondary metabolites
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spelling Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)Cerrado Medicinal plant In vitro culture Secondary metabolitesThis study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 μM) and BAP (0; 4.44; 8.88; 17.75 μM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and absence of light. We obtained friable and compact callus in yellow, green, and red. Culture media containing 4.52 μM 2,4-D + 4.44 μM BAP induced 100% of friable callus with higher fresh and dry weight in the absence of light. The callus produced higher amounts of total phenols and flavonoids than the initial explant. Total tannins were detected only in callus induced in media containing 17.75 μM BAP and 4.52 μM 2,4-D + 17.75 μM BAP, and were not found in the initial explant. The bioactive phenolic compounds detected are derived from benzoic, p-coumaric, cinnamic, gallic acids, and catechins.Revista Ciência Agronômica2015-11-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://periodicos.ufc.br/revistacienciaagronomica/article/view/84604Revista Ciência Agronômica; v. 47 n. 1 (2016)1806-66900045-6888reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFCenghttp://periodicos.ufc.br/revistacienciaagronomica/article/view/84604/228756info:eu-repo/semantics/openAccessCastro, Ana HortênciaBraga, KarinaSousa, FernandaCoimbra, MaironChagas, Rafael César2023-03-01T01:23:24Zoai:periodicos.ufc:article/84604Revistahttps://periodicos.ufc.br/revistacienciaagronomicaPUBhttps://periodicos.ufc.br/revistacienciaagronomica/oai||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2023-03-01T01:23:24Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
title Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
spellingShingle Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
Castro, Ana Hortência
Cerrado
Medicinal plant
In vitro culture
Secondary metabolites
title_short Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
title_full Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
title_fullStr Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
title_full_unstemmed Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
title_sort Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
dc.creator.none.fl_str_mv Castro, Ana Hortência
Braga, Karina
Sousa, Fernanda
Coimbra, Mairon
Chagas, Rafael César
author Castro, Ana Hortência
author_facet Castro, Ana Hortência
Braga, Karina
Sousa, Fernanda
Coimbra, Mairon
Chagas, Rafael César
author_role author
author2 Braga, Karina
Sousa, Fernanda
Coimbra, Mairon
Chagas, Rafael César
author2_role author
author
author
author
dc.subject.por.fl_str_mv Cerrado
Medicinal plant
In vitro culture
Secondary metabolites
topic Cerrado
Medicinal plant
In vitro culture
Secondary metabolites
description This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 μM) and BAP (0; 4.44; 8.88; 17.75 μM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and absence of light. We obtained friable and compact callus in yellow, green, and red. Culture media containing 4.52 μM 2,4-D + 4.44 μM BAP induced 100% of friable callus with higher fresh and dry weight in the absence of light. The callus produced higher amounts of total phenols and flavonoids than the initial explant. Total tannins were detected only in callus induced in media containing 17.75 μM BAP and 4.52 μM 2,4-D + 17.75 μM BAP, and were not found in the initial explant. The bioactive phenolic compounds detected are derived from benzoic, p-coumaric, cinnamic, gallic acids, and catechins.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://periodicos.ufc.br/revistacienciaagronomica/article/view/84604
url http://periodicos.ufc.br/revistacienciaagronomica/article/view/84604
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://periodicos.ufc.br/revistacienciaagronomica/article/view/84604/228756
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista Ciência Agronômica
publisher.none.fl_str_mv Revista Ciência Agronômica
dc.source.none.fl_str_mv Revista Ciência Agronômica; v. 47 n. 1 (2016)
1806-6690
0045-6888
reponame:Revista ciência agronômica (Online)
instname:Universidade Federal do Ceará (UFC)
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instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Revista ciência agronômica (Online)
collection Revista ciência agronômica (Online)
repository.name.fl_str_mv Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv ||alekdutra@ufc.br|| ccarev@ufc.br
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