Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions

Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture...

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Detalles Bibliográficos
Autores: Pieri, Naira Caroline Godoy, de Souza, Aline Fernanda, Botigelli, Ramon Cesar [UNESP], Pessôa, Laís Vicari de Figueiredo, Recchia, Kaiana, Machado, Lucas Simões, Glória, Mayra Hirakawa, de Castro, Raquel Vasconcelos Guimarães [UNESP], Leal, Diego Feitosa, Fantinato Neto, Paulo, Martins, Simone Maria Massami Kitamura, dos Santos Martins, Daniele, Bressan, Fabiana Fernandes, de Andrade, André Furugen Cesar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/207956
Acceso en línea:http://dx.doi.org/10.1007/s12015-021-10198-8
http://hdl.handle.net/11449/207956
Access Level:acceso abierto
Palabra clave:PGCs
Porcine
Regenerative medicine
Reproduction
Translational medicine
Descripción
Sumario:Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.]

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