Hippeastrum reticulatum (Amaryllidaceae) : alkaloid profiling, biological activities and molecular docking

The Amaryllidaceae family has proven to be a rich source of active compounds, which are characterized by unique skeleton arrangements and a broad spectrum of biological activities. The aim of this work was to perform the first detailed study of the alkaloid constituents of Hippeastrum reticulatum (A...

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Detalhes bibliográficos
Autores: Tallini, Luciana Ruschel, Osorio López, Edison Humberto, Santos, Vanessa Dias dos, Borges, Warley de Souza, Kaiser, Marcel, Viladomat, Francesc, Zuanazzi, Jose Angelo Silveira, Bastida, Jaume
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/178504
Acesso em linha:http://hdl.handle.net/10183/178504
Access Level:acceso abierto
Palavra-chave:Amaryllidaceae
6 -hydroxymaritidine
Reticulinine
Isoreticulinine
Hippeastrum reticulatum
Descrição
Resumo:The Amaryllidaceae family has proven to be a rich source of active compounds, which are characterized by unique skeleton arrangements and a broad spectrum of biological activities. The aim of this work was to perform the first detailed study of the alkaloid constituents of Hippeastrum reticulatum (Amaryllidaceae) and to determine the anti-parasitological and cholinesterase (AChE and BuChE) inhibitory activities of the epimers (6 -hydroxymaritidine and 6 -hydroxymaritidine). Twelve alkaloids were identified in H. reticulatum: eight known alkaloids by GC-MS and four unknown (6 -hydroxymaritidine, 6 -hydroxymaritidine, reticulinine and isoreticulinine) by NMR. The epimer mixture (6 -hydroxymaritidine and 6 -hydroxymaritidine) showed low activity against all protozoan parasites tested and weak AChE-inhibitory activity. Finally, a molecular docking analysis of AChE and BuChE proteins showed that isoreticulinine may be classified as a potential inhibitory molecule since it can be stabilized in the active site through hydrogen bonds, - stacking and hydrophobic interactions.