Análise proteômica do plasma de pacientes portadores de hepatocarcinoma

The liver cancer causes death more often than many other tumors because patients are usually diagnosed late. Hepatocellular carcinoma (HCC) is the primary most common type of liver cancer and represents, on average, 80% of all primary types of liver cancer. Thus, it is necessary to search for plasma...

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Detalles Bibliográficos
Autor: Freitas, Melissa Sanders
Tipo de recurso: tesis de maestría
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:portugués
OAI Identifier:oai:repositorio.ufc.br:riufc/29084
Acceso en línea:http://www.repositorio.ufc.br/handle/riufc/29084
Access Level:acceso abierto
Palabra clave:Lectina
Câncer de fígado
Proteômica
Biomarcadores
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network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.pt_BR.fl_str_mv Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
dc.title.en.pt_BR.fl_str_mv Proteomic analysis of plasma of patients with hepatocarcinoma
title Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
spellingShingle Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
Freitas, Melissa Sanders
Lectina
Câncer de fígado
Proteômica
Biomarcadores
title_short Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
title_full Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
title_fullStr Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
title_full_unstemmed Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
title_sort Análise proteômica do plasma de pacientes portadores de hepatocarcinoma
dc.creator.none.fl_str_mv Freitas, Melissa Sanders
author Freitas, Melissa Sanders
author_facet Freitas, Melissa Sanders
author_role author
dc.contributor.advisor1.fl_str_mv Moreira, Renato de Azevedo
contributor_str_mv Moreira, Renato de Azevedo
dc.subject.por.fl_str_mv Lectina
Câncer de fígado
Proteômica
Biomarcadores
topic Lectina
Câncer de fígado
Proteômica
Biomarcadores
description The liver cancer causes death more often than many other tumors because patients are usually diagnosed late. Hepatocellular carcinoma (HCC) is the primary most common type of liver cancer and represents, on average, 80% of all primary types of liver cancer. Thus, it is necessary to search for plasmatic biomarkers for early detection and diagnosis of HCC, method less invasive and easy to obtain. Abnormal glycosylation of some proteins arising from post-translational processing may be an indicator of pathologies, as well as inflammations and malignancies such as cancer. Thus, the glycoproteins are an important subject of study as biomarkers. One of the most used methods for detection of glycoproteins is through the use of lectins, which have high affinity for glycoconjugates. The frutalin, a tetrameric lectin from Artocarpus incisa seeds or "breadfruit", has an affinity for α-D-galactose residues and has been described as a potential lectin in biomedical applications. This paper proposes the search for potential biomarkers in cancer patients livers by comparing between the plasma of healthy individuals and patients with HCC. The plasma collected was imunodepletation and subsequently, serum glycoproteins were fractionated by affinity chromatography on Sepharose 4B matrix with immobilized frutalin, dialyzed, concentrated, and digested with trypsin for identification by mass spectrometry (MS). In total, they identified 19 proteins related directly or indirectly to the HCC and may present as potential biomarkers. Using the methodology engaging affinity chromatography with an immobilized plant lectin column and it was possible to provide a panel of candidate proteins to biological markers to assist in the early identification of patients with HCC, and thus increase the survival chances thereof, as well as to evaluate the use of a plant lectin fractionation of complex samples.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2018-01-18T18:02:59Z
dc.date.available.fl_str_mv 2018-01-18T18:02:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREITAS, Melissa Sanders. Análise proteômica do plasma de pacientes portadores de hepatocarcinoma. 2016. 84 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal do Ceará, Fortaleza, 2016.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/29084
identifier_str_mv FREITAS, Melissa Sanders. Análise proteômica do plasma de pacientes portadores de hepatocarcinoma. 2016. 84 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal do Ceará, Fortaleza, 2016.
url http://www.repositorio.ufc.br/handle/riufc/29084
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/29084/1/2016_dis_msfreitas.pdf
http://repositorio.ufc.br/bitstream/riufc/29084/2/license.txt
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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spelling Moreira, Renato de Azevedo2018-01-18T18:02:59Z2018-01-18T18:02:59Z2016FREITAS, Melissa Sanders. Análise proteômica do plasma de pacientes portadores de hepatocarcinoma. 2016. 84 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal do Ceará, Fortaleza, 2016.http://www.repositorio.ufc.br/handle/riufc/29084The liver cancer causes death more often than many other tumors because patients are usually diagnosed late. Hepatocellular carcinoma (HCC) is the primary most common type of liver cancer and represents, on average, 80% of all primary types of liver cancer. Thus, it is necessary to search for plasmatic biomarkers for early detection and diagnosis of HCC, method less invasive and easy to obtain. Abnormal glycosylation of some proteins arising from post-translational processing may be an indicator of pathologies, as well as inflammations and malignancies such as cancer. Thus, the glycoproteins are an important subject of study as biomarkers. One of the most used methods for detection of glycoproteins is through the use of lectins, which have high affinity for glycoconjugates. The frutalin, a tetrameric lectin from Artocarpus incisa seeds or "breadfruit", has an affinity for α-D-galactose residues and has been described as a potential lectin in biomedical applications. This paper proposes the search for potential biomarkers in cancer patients livers by comparing between the plasma of healthy individuals and patients with HCC. The plasma collected was imunodepletation and subsequently, serum glycoproteins were fractionated by affinity chromatography on Sepharose 4B matrix with immobilized frutalin, dialyzed, concentrated, and digested with trypsin for identification by mass spectrometry (MS). In total, they identified 19 proteins related directly or indirectly to the HCC and may present as potential biomarkers. Using the methodology engaging affinity chromatography with an immobilized plant lectin column and it was possible to provide a panel of candidate proteins to biological markers to assist in the early identification of patients with HCC, and thus increase the survival chances thereof, as well as to evaluate the use of a plant lectin fractionation of complex samples.O câncer hepático causa morte com maior frequência do que muitos outros tumores, pois os pacientes são normalmente diagnosticados tardiamente. O hepatocarcinoma (HCC) é o tipo primário de câncer de fígado mais comum e representa, em média, 80% de todos os tipos primários de câncer de fígado. Desse modo, se faz necessário a busca de biomarcadores plasmáticos para a detecção precoce e diagnóstico da doença, por se tratar de uma técnica menos invasiva e de fácil obtenção. A glicosilação anormal de algumas proteínas decorrente de processamentos pós-traducionais pode ser um indicador de patologias, como também de inflamações e malignidades como o câncer. Sendo assim, as glicoproteínas são um importante alvo de estudo como biomarcadores. Um dos métodos mais utilizados para detecção de glicoproteínas é através do uso de lectinas, que possuem alta afinidade por glicoconjugados. A frutalina, uma lectina tetramérica isolada a partir de sementes de Artocarpus incisa ou “fruta-pão”, apresenta afinidade por resíduos de α-D-galactose e já foi descrita como uma lectina potencial em aplicações biomédicas. O presente trabalho propõe a busca de potenciais biomarcadores em pacientes com câncer de fígados através da comparação entre o plasma de indivíduos saudáveis e de pacientes com HCC. O plasma coletado foi imunodepletado e, posteriormente, as glicoproteínas séricas foram fracionadas por meio de cromatografia de afinidade em matriz de Sepharose 4B imobilizada com frutalina. Em seguida, as amostras foram dialisadas, concentradas e digeridas com tripsina para identificação por Espectrometria de Massas (EM). No total foram identificadas 19 proteínas relacionadas diretamente ou indiretamente com o HCC e que podem se apresentar como potenciais biomarcadores. Através da metodologia que acopla a cromatografia de afinidade com uma lectina vegetal imobilizada na matrix cromatográfica e EM, foi possível apresentar um painel de proteínas candidatas a marcadores biológicos para auxiliar a identificação precoce de pacientes com HCC, e assim, aumentar a chances de sobrevida destes, como também avaliar a utilização de uma lectina vegetal no fracionamento de amostras protéicas complexas.LectinaCâncer de fígadoProteômicaBiomarcadoresAnálise proteômica do plasma de pacientes portadores de hepatocarcinomaProteomic analysis of plasma of patients with hepatocarcinomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessFreitas, Melissa SandersORIGINAL2016_dis_msfreitas.pdf2016_dis_msfreitas.pdfapplication/pdf1113966http://repositorio.ufc.br/bitstream/riufc/29084/1/2016_dis_msfreitas.pdf40bfbd628014a3c56dbfa63e287254baMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/29084/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufc/290842020-05-25 16:12:03.152oai:repositorio.ufc.br:riufc/29084Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2020-05-25T19:12:03Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
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