Estudo comparativo dos métodos diagnósticos para leishmaniose visceral em cães oriundos de Ilha Solteira, SP

The purpose of the present work was a comparative study of diagnostic methods for Canine Visceral Leishmaniasis (CVL) using serological methods, enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT), histochemical (HE) and immunohistochemical (IMHC) tests using sple...

Descripción completa

Detalles Bibliográficos
Autores: de Assis, Juliana [UNESP], de Queiroz, Nina Marí Gual Pimenta [UNESP], da Silveira, Rita de Cássia Viveiros [UNESP], Nunes, Cáris Maroni [UNESP], Oliveira, Trícia Maria Ferreira de Sousa [UNESP], de Noronha Junior, Antonio Carlos Faconti, Neves, Maria Francisca [UNESP], Machado, Rosangela Zacarias [UNESP], Buzetti, Wilma Aparecida Starke [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:portugués
OAI Identifier:oai:repositorio.unesp.br:11449/225875
Acceso en línea:http://hdl.handle.net/11449/225875
Access Level:acceso abierto
Palabra clave:ELISA
IFAT
Immunohistochemistry
Leishmania (L.) chagasi
PCR
Descripción
Sumario:The purpose of the present work was a comparative study of diagnostic methods for Canine Visceral Leishmaniasis (CVL) using serological methods, enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT), histochemical (HE) and immunohistochemical (IMHC) tests using spleen, lymph node and liver canine tissues. In addition, Polymerase Chain Reaction (PCR) was done in blood and in tissues in order to compare and confirm no conclusive and negative diagnosis by the methods above. For this study, 34 dogs were divided according to clinical signs in asymptomatic, oligosymptomatic and polisymptomatic Leishmania-infected dogs euthanized by Zoonotic Disease Control Center (CCZ) from Ilha Solteira, SP, Brazil. The positivism indexes of ELISA, IMHC, IFAT and HE were 65.0, 62.0, 56.0 and 56.0%, respectively with the highest numbers of positive dogs in polisymptomatic (92.0%) followed by oligosymptomatic (57.0%) and asymptomatic dogs (12.5%). Furthermore, PCR confirmed the positive results and detected DNA in tissues from 100% of negative dogs and 89.0% suspects raising the animal positivism index up to 97.0%. In conclusion, PCR was the most sensitive and a valuable method for a definitive CVL diagnosis.

Similares en LA Referencia