Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model
Background and Purpose Asthma is an inflammatory disease that involves airway hyperresponsiveness and remodelling. Flavonoids have been associated to anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment of asthma. Our aim was to evaluate the effects of the...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2013 |
| País: | Brasil |
| Recursos: | Universidade Federal de São Paulo (UNIFESP) |
| Repositório: | Repositório Institucional da UNIFESP |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/36116 |
| Acesso em linha: | https://dx.doi.org/10.1111/bph.12062 https://repositorio.unifesp.br/handle/11600/36116 |
| Access Level: | Acceso aberto |
| Palavra-chave: | sakuranetin Experimental asthma model Airway remodelling Cytokines Oxidative stress |
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Flavonone treatment reverses airway inflammation and remodelling in an asthma murine modelsakuranetinExperimental asthma modelAirway remodellingCytokinesOxidative stressBackground and Purpose Asthma is an inflammatory disease that involves airway hyperresponsiveness and remodelling. Flavonoids have been associated to anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment of asthma. Our aim was to evaluate the effects of the sakuranetin treatment in several aspects of experimental asthma model in mice. Experimental Approach Male BALB/c mice received ovalbumin (i.p.) on days 0 and 14, and were challenged with aerolized ovalbumin 1% on days 24, 26 and 28. Ovalbumin-sensitized animals received vehicle (saline and dimethyl sulfoxide, DMSO), sakuranetin (20mg kg1 per mice) or dexamethasone (5mg kg1 per mice) daily beginning from 24th to 29th day. Control group received saline inhalation and nasal drop vehicle. On day 29, we determined the airway hyperresponsiveness, inflammation and remodelling as well as specific IgE antibody. RANTES, IL-5, IL-4, Eotaxin, IL-10, TNF-, IFN- and GMC-SF content in lung homogenate was performed by Bioplex assay, and 8-isoprostane and NF-kB activations were visualized in inflammatory cells by immunohistochemistry. Key Results We have demonstrated that sakuranetin treatment attenuated airway hyperresponsiveness, inflammation and remodelling; and these effects could be attributed to Th2 pro-inflammatory cytokines and oxidative stress reduction as well as control of NF-kB activation. Conclusions and Implications These results highlighted the importance of counteracting oxidative stress by flavonoids in this asthma model and suggest sakuranetin as a potential candidate for studies of treatment of asthma.Univ São Paulo, Sch Med, Dept Med, São Paulo, BrazilUniv São Paulo, Inst Biol, Dept Imunol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, BrazilUniversidade Federal de São Paulo, Diadema, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, BrazilUniversidade Federal de São Paulo, Diadema, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Wiley-BlackwellUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:31:27Z2016-01-24T14:31:27Z2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1736-1749https://dx.doi.org/10.1111/bph.12062British Journal of Pharmacology. Hoboken: Wiley-Blackwell, v. 168, n. 7, p. 1736-1749, 2013.10.1111/bph.120620007-1188https://repositorio.unifesp.br/handle/11600/36116WOS:000316262500016ark:/48912/0013000025rpjengBritish Journal of Pharmacologyinfo:eu-repo/semantics/openAccesshttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPToledo, Alessandra ChoquetaSakoda, Camila Pivari Pedroso [UNIFESP]Perini, AdenirPinheiro, Nathalia MontouroMagalhaes, Renato MoschellaGrecco, Simone dos Santos [UNIFESP]Tiberio, Iolanda de Fátima Lopes CalvoCamara, Niels Olsen SaraivaMartins, Milton de ArrudaLago, Joao Henrique Ghilardi [UNIFESP]Prado, Carla Máximo [UNIFESP]2021-11-16T16:59:20Zoai:repositorio.unifesp.br:11600/36116Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-11-16T16:59:20Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| title |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| spellingShingle |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model Toledo, Alessandra Choqueta sakuranetin Experimental asthma model Airway remodelling Cytokines Oxidative stress |
| title_short |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| title_full |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| title_fullStr |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| title_full_unstemmed |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| title_sort |
Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model |
| dc.creator.none.fl_str_mv |
Toledo, Alessandra Choqueta Sakoda, Camila Pivari Pedroso [UNIFESP] Perini, Adenir Pinheiro, Nathalia Montouro Magalhaes, Renato Moschella Grecco, Simone dos Santos [UNIFESP] Tiberio, Iolanda de Fátima Lopes Calvo Camara, Niels Olsen Saraiva Martins, Milton de Arruda Lago, Joao Henrique Ghilardi [UNIFESP] Prado, Carla Máximo [UNIFESP] |
| author |
Toledo, Alessandra Choqueta |
| author_facet |
Toledo, Alessandra Choqueta Sakoda, Camila Pivari Pedroso [UNIFESP] Perini, Adenir Pinheiro, Nathalia Montouro Magalhaes, Renato Moschella Grecco, Simone dos Santos [UNIFESP] Tiberio, Iolanda de Fátima Lopes Calvo Camara, Niels Olsen Saraiva Martins, Milton de Arruda Lago, Joao Henrique Ghilardi [UNIFESP] Prado, Carla Máximo [UNIFESP] |
| author_role |
author |
| author2 |
Sakoda, Camila Pivari Pedroso [UNIFESP] Perini, Adenir Pinheiro, Nathalia Montouro Magalhaes, Renato Moschella Grecco, Simone dos Santos [UNIFESP] Tiberio, Iolanda de Fátima Lopes Calvo Camara, Niels Olsen Saraiva Martins, Milton de Arruda Lago, Joao Henrique Ghilardi [UNIFESP] Prado, Carla Máximo [UNIFESP] |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
| dc.subject.por.fl_str_mv |
sakuranetin Experimental asthma model Airway remodelling Cytokines Oxidative stress |
| topic |
sakuranetin Experimental asthma model Airway remodelling Cytokines Oxidative stress |
| description |
Background and Purpose Asthma is an inflammatory disease that involves airway hyperresponsiveness and remodelling. Flavonoids have been associated to anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment of asthma. Our aim was to evaluate the effects of the sakuranetin treatment in several aspects of experimental asthma model in mice. Experimental Approach Male BALB/c mice received ovalbumin (i.p.) on days 0 and 14, and were challenged with aerolized ovalbumin 1% on days 24, 26 and 28. Ovalbumin-sensitized animals received vehicle (saline and dimethyl sulfoxide, DMSO), sakuranetin (20mg kg1 per mice) or dexamethasone (5mg kg1 per mice) daily beginning from 24th to 29th day. Control group received saline inhalation and nasal drop vehicle. On day 29, we determined the airway hyperresponsiveness, inflammation and remodelling as well as specific IgE antibody. RANTES, IL-5, IL-4, Eotaxin, IL-10, TNF-, IFN- and GMC-SF content in lung homogenate was performed by Bioplex assay, and 8-isoprostane and NF-kB activations were visualized in inflammatory cells by immunohistochemistry. Key Results We have demonstrated that sakuranetin treatment attenuated airway hyperresponsiveness, inflammation and remodelling; and these effects could be attributed to Th2 pro-inflammatory cytokines and oxidative stress reduction as well as control of NF-kB activation. Conclusions and Implications These results highlighted the importance of counteracting oxidative stress by flavonoids in this asthma model and suggest sakuranetin as a potential candidate for studies of treatment of asthma. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-04-01 2016-01-24T14:31:27Z 2016-01-24T14:31:27Z |
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info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1111/bph.12062 British Journal of Pharmacology. Hoboken: Wiley-Blackwell, v. 168, n. 7, p. 1736-1749, 2013. 10.1111/bph.12062 0007-1188 https://repositorio.unifesp.br/handle/11600/36116 WOS:000316262500016 |
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ark:/48912/0013000025rpj |
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https://dx.doi.org/10.1111/bph.12062 https://repositorio.unifesp.br/handle/11600/36116 |
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British Journal of Pharmacology. Hoboken: Wiley-Blackwell, v. 168, n. 7, p. 1736-1749, 2013. 10.1111/bph.12062 0007-1188 WOS:000316262500016 ark:/48912/0013000025rpj |
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eng |
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eng |
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British Journal of Pharmacology |
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info:eu-repo/semantics/openAccess http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
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openAccess |
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1736-1749 |
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Wiley-Blackwell |
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Wiley-Blackwell |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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