Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients

Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Fiv...

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Detalles Bibliográficos
Autores: Damasceno, Lisandra Serra, Vite-Garín, Tania, Ramírez, José Antonio, Rodríguez-Arellanes, Gabriela, Almeida, Marcos Abreu de, Muniz, Mauro de Medeiros, Mesquita, Jacó Ricarte Lima de, Leitão, Terezinha do Menino Jesus Silva, Taylor, Maria Lucia, Zancopé-Oliveira, Rosely Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:inglés
OAI Identifier:oai:repositorio.ufc.br:riufc/46425
Acceso en línea:http://www.repositorio.ufc.br/handle/riufc/46425
Access Level:acceso abierto
Palabra clave:Coinfecção
Coinfection
Histoplasma
Histoplasmose
Histoplasmosis
HIV
Descripción
Sumario:Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.

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