Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection

We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Est...

Descripción completa

Detalles Bibliográficos
Autores: Fonseca, Denise Morais da, Silva, Célio Lopes, Paula, Marina Oliveira e, Soares, Edson Garcia, Marchal, Gilles, Horn, Cynthia, Bonato, Vânia Luiza Deperon
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/57312
Acceso en línea:https://arca.fiocruz.br/handle/icict/57312
Access Level:acceso abierto
Palabra clave:Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
03 Saúde e Bem-Estar
id BR_a8c4629d90a89febbf64bc8d1e4a464d
oai_identifier_str oai:arca.fiocruz.br:icict/57312
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
spellingShingle Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
Fonseca, Denise Morais da
Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
03 Saúde e Bem-Estar
title_short Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_full Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_fullStr Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_full_unstemmed Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_sort Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
dc.creator.none.fl_str_mv Fonseca, Denise Morais da
Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
author Fonseca, Denise Morais da
author_facet Fonseca, Denise Morais da
Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
author_role author
author2 Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
author2_role author
author
author
author
author
author
dc.subject.en.none.fl_str_mv Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
topic Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
03 Saúde e Bem-Estar
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
description We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the State of Sa˜o Paulo; grant no. 03/11303-2 and 00/09663-2), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq, Council for Scientific and Technological Development), and the Rede Brasileira de Pesquisa em TB (Rede-TB, Brazilian Tuberculosis Research Network).
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2023-03-09T02:44:00Z
dc.date.available.fl_str_mv 2023-03-09T02:44:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/57312
dc.identifier.issn.none.fl_str_mv 1365-2567
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2567.2007.02597.x
dc.identifier.eissn.none.fl_str_mv 1365-2567
identifier_str_mv FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.
1365-2567
10.1111/j.1365-2567.2007.02597.x
url https://arca.fiocruz.br/handle/icict/57312
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://arca.fiocruz.br/bitstreams/d7d045c4-5e1b-45d7-91fc-c2e33e2b3a1f/download
https://arca.fiocruz.br/bitstreams/303d3d75-554c-43b7-b964-9720b4925f3d/download
https://arca.fiocruz.br/bitstreams/9a516282-7851-4988-a1bd-9a0a5d71430a/download
https://arca.fiocruz.br/bitstreams/c296d55a-52dd-4bb1-a461-7f49b7181bf2/download
bitstream.checksum.fl_str_mv d1b636a5d4010982cf49fab73ed2a6c3
8a4605be74aa9ea9d79846c1fba20a33
2e0f1bfd3173780e4b105ac366e52856
8ae6452512568e11ad27df5d2363fc6c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1853674408661483520
spelling 2023-03-09T02:44:00Z2023-03-09T02:44:00Z2007FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.1365-2567https://arca.fiocruz.br/handle/icict/5731210.1111/j.1365-2567.2007.02597.x1365-2567We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the State of Sa˜o Paulo; grant no. 03/11303-2 and 00/09663-2), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq, Council for Scientific and Technological Development), and the Rede Brasileira de Pesquisa em TB (Rede-TB, Brazilian Tuberculosis Research Network).University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Pathology. Ribeirão Preto, SP, Brazil.Institute Pasteur. Laboratoire de Référence des Mycobacteries. Paris, France.Oswaldo Cruz Foundation. Evandro Chagas Clinical Research Institute. Rio de Janeiro, RJ, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.The results of various animal model studies of tuberculosis (TB) suggest that culture filtrate proteins (CFPs), which are antigens secreted by Mycobacterium tuberculosis, are largely responsible for improvements in TB vaccines. The great obstacle to developing protein subunit vaccines is that adjuvants are required in order to stimulate relevant protective immune responses. Acting as immune adjuvants, CpG-oligodeoxynucleotides (CpG-ODNs) promote the activation of Th1 cells and of pro-inflammatory cytokines. To evaluate the adjuvant role of CpG-ODNs in conferring enhanced immunogenic capacity and protection against M. tuberculosis, we immunized mice with CFP antigen combined with synthetic CpG-ODNs (CFP/CpG) or with incomplete Freund's adjuvant (IFA) (CFP/IFA). Immunization with CFP/CpG induced a T helper 1 (Th1)-biased response accompanied by a higher immunoglobulin G2a (IgG2a) antibody/IgG1 antibody ratio, elevated production of interferon-gamma (IFN-gamma) by spleen cells and in lungs. However, CFP/IFA-immunized mice presented higher levels of IgG1 antibodies, as well as increased production of IFN-gamma, interleukin (IL)-5, and IL-10 by spleen cells, together with lower levels of IFN-gamma in the lungs. Despite the stronger Th1 response seen in both groups, believed to be necessary for protection against TB, only mice immunized with CFP/IFA were protected after M. tuberculosis infection. Lung histology revealed that lung parenchyma were better preserved in CFP/IFA-immunized mice, which also presented intense lymphocyte recruitment to the lesion, whereas CFP/CpG-immunized mice presented severe pulmonary injury accompanied by necrosis. Based on the data presented, we discuss the widely accepted paradigm that high levels of IFN-gamma are directly correlated with protection against experimental TB.engWiley Online LibraryIncreased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTuberculosisCulture filtrate proteinsCpG oligodeoxynucleotidesVaccineAdjuvant03 Saúde e Bem-Estarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZFonseca, Denise Morais daSilva, Célio LopesPaula, Marina Oliveira eSoares, Edson GarciaMarchal, GillesHorn, CynthiaBonato, Vânia Luiza DeperonORIGINALve_Fonseca_Denise_etal_INI_2007.pdfve_Fonseca_Denise_etal_INI_2007.pdfapplication/pdf604897https://arca.fiocruz.br/bitstreams/d7d045c4-5e1b-45d7-91fc-c2e33e2b3a1f/downloadd1b636a5d4010982cf49fab73ed2a6c3MD52trueAnonymousREADLICENSElicense.txttext/plain1748https://arca.fiocruz.br/bitstreams/303d3d75-554c-43b7-b964-9720b4925f3d/download8a4605be74aa9ea9d79846c1fba20a33MD51falseAnonymousREADTEXTve_Fonseca_Denise_etal_INI_2007.pdf.txtve_Fonseca_Denise_etal_INI_2007.pdf.txtExtracted texttext/plain46391https://arca.fiocruz.br/bitstreams/9a516282-7851-4988-a1bd-9a0a5d71430a/download2e0f1bfd3173780e4b105ac366e52856MD511falseAnonymousREADTHUMBNAILve_Fonseca_Denise_etal_INI_2007.pdf.jpgve_Fonseca_Denise_etal_INI_2007.pdf.jpgGenerated Thumbnailimage/jpeg46116https://arca.fiocruz.br/bitstreams/c296d55a-52dd-4bb1-a461-7f49b7181bf2/download8ae6452512568e11ad27df5d2363fc6cMD512falseAnonymousREADicict/573122025-12-10 13:00:01.03open.accessoai:arca.fiocruz.br:icict/57312https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T16:00:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
score 15,301603

Similares en LA Referencia