Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
This work aims to develop analytical methods using high-performance liquid chromatographywith a diode array detector (HPLC-DAD) for analysis and quantification of avermectins(AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized andsome parameters such as temperature, flow...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade de São Paulo (USP) |
| Repositorio: | Brazilian Journal of Pharmaceutical Sciences |
| Idioma: | inglés |
| OAI Identifier: | oai:revistas.usp.br:article/204683 |
| Acceso en línea: | https://www.revistas.usp.br/bjps/article/view/204683 |
| Access Level: | acceso abierto |
| Palabra clave: | Avermectins Milbemycins Quality control Bulk samples |
| Sumario: | This work aims to develop analytical methods using high-performance liquid chromatographywith a diode array detector (HPLC-DAD) for analysis and quantification of avermectins(AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized andsome parameters such as temperature, flow rate, injection volume and mobile phase withdifferent proportions of solvents were evaluated. The best chromatographic conditions wereobtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20°C, flow rate of 1.2 mL min–1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile:ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, andacetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Underthese conditions satisfactory results were obtained, with appropriate limits of detection andquantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfythe need for analytical methods for the multi-determination of MBMs and the B1a and B1bforms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative andshould aid in the development of the field. |
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