Immunometabolic crosstalk in Aedes fluviatilis and Wolbachia pipientis symbiosis

Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host’s physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes fluvi...

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Detalles Bibliográficos
Autores: Silva, Jhenifer N., Calixto, Christiano, Brito, Gisely Cristina Ramos de, Daumas Filho, Carlos Renato de Oliveira, Silva, Ana Beatriz Walter Nuno da, Cunha, Octávio Augusto Talyuli da, Sampaio, Angélica Fernandes Arcanjo, Oliveira, Pedro Lagerblad de, Moreira, Luciano A., Vaz Junior, Itabajara da Silva, Logullo, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/278456
Acceso en línea:http://hdl.handle.net/10183/278456
Access Level:acceso abierto
Palabra clave:Wolbachia
Glicogênio sintase quinase 3 beta
Transcrição genética
Metabolismo dos carboidratos
Respostas imunes
Células embrionárias
Aedes fluviatilis
Symbiosis
Immunometabolism
GSK3β
Relish2
Descripción
Sumario:Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host’s physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes fluviatilis (Ae. fluviatilis). Glycogen synthesis is controlled by the enzyme GSK3, which is also involved in immune responses in both vertebrate and invertebrate organisms. Here we investigated the mechanisms behind immune changes mediated by glycogen synthase kinase β (GSK3β) in the symbiosis between Ae. fluviatilis and W. pipientis using a GSK3β inhibitor or RNAi-mediated gene silencing. GSK3β inhibition or knockdown increased glycogen content and Wolbachia population, together with a reduction in Relish2 and gambicin transcripts. Furthermore, knockdown of Relish2 or Caspar revealed that the immunodeficiency pathway acts to control Wolbachia numbers in the host. In conclusion, we describe for the first time the involvement of GSK3β in Ae. fluviatilis imune response, acting to control the Wolbachia endosymbiotic population.

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