Yellow fever virus genotyping tool and investigation of suspected adverse events following yellow fever vaccination
The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of Y...
| Autores: | , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/80160 |
| Acceso en línea: | https://doi.org/10.3390/vaccines7040206 http://hdl.handle.net/1843/80160 https://orcid.org/0000-0003-2616-6300 https://orcid.org/0000-0002-9659-5621 https://orcid.org/0000-0001-5869-2183 https://orcid.org/0000-0002-4256-247X https://orcid.org/0000-0002-5426-0655 https://orcid.org/0000-0001-6306-3605 https://orcid.org/0000-0002-1208-3179 https://orcid.org/0000-0002-0487-8748 https://orcid.org/0000-0002-1406-6401 https://orcid.org/0000-0003-2200-6166 https://orcid.org/0000-0003-2721-3826 https://orcid.org/0000-0002-5494-4889 https://orcid.org/0000-0003-2814-5183 https://orcid.org/0000-0002-4049-4055 |
| Access Level: | acceso abierto |
| Palabra clave: | Yellow Fever virus YFV-17DD Adverse event Yellow Fever Vaccination Genotyping Wild-type virus Vaccine Arboviruses Immunization Febre amarela Vírus da Febre Amarela Vacinação Imunização Infecções por Arbovirus Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos |
| Sumario: | The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 50UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes. |
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