Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)

A cDNA phage library was constructed from venom glands of a single adult specimen of crotamine-plus Crotalus durissus terri®cus (South American rattlesnake) captured in a known region. Fifteen crotamine positive clones were isolated using a PCR-based screening protocol and sequenced. These complete...

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Authors: Rádis-Baptista, Gandhi, Oguiura, Nancy, Hayashi, M.A.F., Camargoc, M.E., Gregod, K.F., Oliveira, E.B., Yamane, T.
Format: article
Status:Published version
Publication Date:1999
Country:Brasil
Institution:Universidade Federal do Ceará (UFC)
Repository:Repositório Institucional da Universidade Federal do Ceará (UFC)
Language:English
OAI Identifier:oai:repositorio.ufc.br:riufc/59788
Online Access:http://www.repositorio.ufc.br/handle/riufc/59788
Access Level:Open access
Keyword:Veneno
Cobras
Peptídeos
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network_name_str Brasil
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dc.title.pt_BR.fl_str_mv Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
dc.title.en.pt_BR.fl_str_mv Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
title Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
spellingShingle Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
Rádis-Baptista, Gandhi
Veneno
Cobras
Peptídeos
title_short Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
title_full Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
title_fullStr Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
title_full_unstemmed Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
title_sort Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)
dc.creator.none.fl_str_mv Rádis-Baptista, Gandhi
Oguiura, Nancy
Hayashi, M.A.F.
Camargoc, M.E.
Gregod, K.F.
Oliveira, E.B.
Yamane, T.
author Rádis-Baptista, Gandhi
author_facet Rádis-Baptista, Gandhi
Oguiura, Nancy
Hayashi, M.A.F.
Camargoc, M.E.
Gregod, K.F.
Oliveira, E.B.
Yamane, T.
author_role author
author2 Oguiura, Nancy
Hayashi, M.A.F.
Camargoc, M.E.
Gregod, K.F.
Oliveira, E.B.
Yamane, T.
author2_role author
author
author
author
author
author
dc.subject.por.fl_str_mv Veneno
Cobras
Peptídeos
topic Veneno
Cobras
Peptídeos
description A cDNA phage library was constructed from venom glands of a single adult specimen of crotamine-plus Crotalus durissus terri®cus (South American rattlesnake) captured in a known region. Fifteen crotamine positive clones were isolated using a PCR-based screening protocol and sequenced. These complete cDNAs clones were grouped for maximal alignment into six distinct nucleotide sequences. The crotamine cDNAs, with 340±360 bases, encompass open reading frame of 198 nucleotides with 50 and 30 untranslated regions of variable size, signal peptide sequence, one crotamine isoform message, and putative poly(A + ) signal. Of these six di erent crotamine cDNA precursors, two predict the identical amino acid sequence previously described by Laure (1975), and the other four a crotamine isoform precursor where the Leucine residue at position 19 is replaced by isoleucine by a single base change. On the other hand, nucleotide variation was observed in the 50 and 30 untranslated regions, with one interesting variant containing an 18 base pair deletion at the 50 untranslated region which results in the usual ATG initiator being replaced by the rarely used GUG start codon. Comparison by Northern blot analysis of poly(A + ) RNA from venom glands of a crotamine-plus specimen to total and poly(A + ) RNA from a crotamine-minus snake
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2021-07-30T14:12:37Z
dc.date.available.fl_str_mv 2021-07-30T14:12:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RÁDIS-BAPTISTA, G.; OGUIURA, N.; HAYASHI, M.A.F.; CAMARGOC, M.E., Gregod, K.F.; OLIVEIRA, E.B.; YAMANE, T.. Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake (Crotalus durissus terrificus). Toxicon, United Kingdom, v. 37, p. 973-984. 1999.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/59788
dc.identifier.issn.none.fl_str_mv 0041-0101
identifier_str_mv RÁDIS-BAPTISTA, G.; OGUIURA, N.; HAYASHI, M.A.F.; CAMARGOC, M.E., Gregod, K.F.; OLIVEIRA, E.B.; YAMANE, T.. Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake (Crotalus durissus terrificus). Toxicon, United Kingdom, v. 37, p. 973-984. 1999.
0041-0101
url http://www.repositorio.ufc.br/handle/riufc/59788
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Toxicon
publisher.none.fl_str_mv Toxicon
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/59788/1/1999_art_gradisbaptista.pdf
http://repositorio.ufc.br/bitstream/riufc/59788/2/license.txt
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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spelling 2021-07-30T14:12:37Z2021-07-30T14:12:37Z1999RÁDIS-BAPTISTA, G.; OGUIURA, N.; HAYASHI, M.A.F.; CAMARGOC, M.E., Gregod, K.F.; OLIVEIRA, E.B.; YAMANE, T.. Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake (Crotalus durissus terrificus). Toxicon, United Kingdom, v. 37, p. 973-984. 1999.0041-0101http://www.repositorio.ufc.br/handle/riufc/59788A cDNA phage library was constructed from venom glands of a single adult specimen of crotamine-plus Crotalus durissus terri®cus (South American rattlesnake) captured in a known region. Fifteen crotamine positive clones were isolated using a PCR-based screening protocol and sequenced. These complete cDNAs clones were grouped for maximal alignment into six distinct nucleotide sequences. The crotamine cDNAs, with 340±360 bases, encompass open reading frame of 198 nucleotides with 50 and 30 untranslated regions of variable size, signal peptide sequence, one crotamine isoform message, and putative poly(A + ) signal. Of these six di erent crotamine cDNA precursors, two predict the identical amino acid sequence previously described by Laure (1975), and the other four a crotamine isoform precursor where the Leucine residue at position 19 is replaced by isoleucine by a single base change. On the other hand, nucleotide variation was observed in the 50 and 30 untranslated regions, with one interesting variant containing an 18 base pair deletion at the 50 untranslated region which results in the usual ATG initiator being replaced by the rarely used GUG start codon. Comparison by Northern blot analysis of poly(A + ) RNA from venom glands of a crotamine-plus specimen to total and poly(A + ) RNA from a crotamine-minus snakeToxiconVenenoCobrasPeptídeosNucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake ( Crotalus durissus terrificus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessRádis-Baptista, GandhiOguiura, NancyHayashi, M.A.F.Camargoc, M.E.Gregod, K.F.Oliveira, E.B.Yamane, T.ORIGINAL1999_art_gradisbaptista.pdf1999_art_gradisbaptista.pdfapplication/pdf567039http://repositorio.ufc.br/bitstream/riufc/59788/1/1999_art_gradisbaptista.pdf868724fe25327b82a1cfc5eb67b3c6abMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/59788/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufc/597882022-11-30 11:14:35.071oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2022-11-30T14:14:35Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
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