A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum

The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spe...

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Autores: Farias, Eduardo Henrique Cunha de [UNIFESP], Pomin, Vitor Hugo, Valente, Ana-Paula, Nader, Helena Bociani [UNIFESP], Rocha, Hugo Alexandre de Oliveira, Mourao, Paulo Antonio de Souza
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/30456
Acceso en línea:https://dx.doi.org/10.1093/glycob/cwm139
https://repositorio.unifesp.br/handle/11600/30456
Access Level:acceso abierto
Palabra clave:Codium isthmocladum
Green alga
Green seaweed
Phylogeny
Pyruvylated-sulfated galactan
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spelling A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladumCodium isthmocladumGreen algaGreen seaweedPhylogenyPyruvylated-sulfated galactanThe green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.Univ Fed Rio Grande do Norte, Dept Bioquim, BIOPOL, Lab Biotechnol Polimeros Nat, BR-59072970 Natal, RN, BrazilHosp Univ Clementino Fraga Filho, Lab Tecido Conjuntivo, Natal, RN, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Ctr Ciencias Saude, BR-21941590 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Ctr Nacl Ressonancia Magnet Nucl Jiri Jonas, BR-21941590 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of ScienceOxford Univ Press IncUniv Fed Rio Grande do NorteHosp Univ Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:49:35Z2016-01-24T13:49:35Z2008-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion250-259https://dx.doi.org/10.1093/glycob/cwm139Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.10.1093/glycob/cwm1390959-6658https://repositorio.unifesp.br/handle/11600/30456WOS:000253832400007ark:/48912/001300001njcnengGlycobiologyinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPFarias, Eduardo Henrique Cunha de [UNIFESP]Pomin, Vitor HugoValente, Ana-PaulaNader, Helena Bociani [UNIFESP]Rocha, Hugo Alexandre de OliveiraMourao, Paulo Antonio de Souza2024-01-19T19:28:16Zoai:repositorio.unifesp.br:11600/30456Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-01-19T19:28:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
spellingShingle A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
Farias, Eduardo Henrique Cunha de [UNIFESP]
Codium isthmocladum
Green alga
Green seaweed
Phylogeny
Pyruvylated-sulfated galactan
title_short A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_full A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_fullStr A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_full_unstemmed A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_sort A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
dc.creator.none.fl_str_mv Farias, Eduardo Henrique Cunha de [UNIFESP]
Pomin, Vitor Hugo
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo Alexandre de Oliveira
Mourao, Paulo Antonio de Souza
author Farias, Eduardo Henrique Cunha de [UNIFESP]
author_facet Farias, Eduardo Henrique Cunha de [UNIFESP]
Pomin, Vitor Hugo
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo Alexandre de Oliveira
Mourao, Paulo Antonio de Souza
author_role author
author2 Pomin, Vitor Hugo
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo Alexandre de Oliveira
Mourao, Paulo Antonio de Souza
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Rio Grande do Norte
Hosp Univ Clementino Fraga Filho
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
dc.subject.por.fl_str_mv Codium isthmocladum
Green alga
Green seaweed
Phylogeny
Pyruvylated-sulfated galactan
topic Codium isthmocladum
Green alga
Green seaweed
Phylogeny
Pyruvylated-sulfated galactan
description The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.
publishDate 2008
dc.date.none.fl_str_mv 2008-03-01
2016-01-24T13:49:35Z
2016-01-24T13:49:35Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1093/glycob/cwm139
Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.
10.1093/glycob/cwm139
0959-6658
https://repositorio.unifesp.br/handle/11600/30456
WOS:000253832400007
dc.identifier.dark.fl_str_mv ark:/48912/001300001njcn
url https://dx.doi.org/10.1093/glycob/cwm139
https://repositorio.unifesp.br/handle/11600/30456
identifier_str_mv Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.
10.1093/glycob/cwm139
0959-6658
WOS:000253832400007
ark:/48912/001300001njcn
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Glycobiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 250-259
dc.publisher.none.fl_str_mv Oxford Univ Press Inc
publisher.none.fl_str_mv Oxford Univ Press Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15.127369

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