HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA

Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Rio de Janeiro, RJ, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.

Detalles Bibliográficos
Autores: Brito, Sieberth Nascimento, Zukurov, Jean Paulo Lopes, Maricato, Juliana Terzi, Volpini, Angela Cristina, Salim, Anna Christina de Matos, Araújo, Flávio Marcos Gomes, Coimbra, Roney Santos, Oliveira, Guilherme Corrêa de, Antoneli, Fernando, Janini, Luiz Mário Ramos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/13545
Acceso en línea:https://arca.fiocruz.br/handle/icict/13545
Access Level:acceso abierto
Palabra clave:HIV-1
Nucleotide sequencing
Coreceptors
Amino acid substitution
Reverse transcription
Substitution mutation
Viral genomics
Host cells
id BR_9f2540c80bcdfa4ef4ccc357cc839f4e
oai_identifier_str oai:arca.fiocruz.br:icict/13545
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
title HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
spellingShingle HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
Brito, Sieberth Nascimento
HIV-1
Nucleotide sequencing
Coreceptors
Amino acid substitution
Reverse transcription
Substitution mutation
Viral genomics
Host cells
title_short HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
title_full HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
title_fullStr HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
title_full_unstemmed HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
title_sort HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA
dc.creator.none.fl_str_mv Brito, Sieberth Nascimento
Zukurov, Jean Paulo Lopes
Maricato, Juliana Terzi
Volpini, Angela Cristina
Salim, Anna Christina de Matos
Araújo, Flávio Marcos Gomes
Coimbra, Roney Santos
Oliveira, Guilherme Corrêa de
Antoneli, Fernando
Janini, Luiz Mário Ramos
author Brito, Sieberth Nascimento
author_facet Brito, Sieberth Nascimento
Zukurov, Jean Paulo Lopes
Maricato, Juliana Terzi
Volpini, Angela Cristina
Salim, Anna Christina de Matos
Araújo, Flávio Marcos Gomes
Coimbra, Roney Santos
Oliveira, Guilherme Corrêa de
Antoneli, Fernando
Janini, Luiz Mário Ramos
author_role author
author2 Zukurov, Jean Paulo Lopes
Maricato, Juliana Terzi
Volpini, Angela Cristina
Salim, Anna Christina de Matos
Araújo, Flávio Marcos Gomes
Coimbra, Roney Santos
Oliveira, Guilherme Corrêa de
Antoneli, Fernando
Janini, Luiz Mário Ramos
author2_role author
author
author
author
author
author
author
author
author
dc.subject.en.none.fl_str_mv HIV-1
Nucleotide sequencing
Coreceptors
Amino acid substitution
Reverse transcription
Substitution mutation
Viral genomics
Host cells
topic HIV-1
Nucleotide sequencing
Coreceptors
Amino acid substitution
Reverse transcription
Substitution mutation
Viral genomics
Host cells
description Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Rio de Janeiro, RJ, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-05T18:20:47Z
dc.date.available.fl_str_mv 2016-04-05T18:20:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITO, Sieberth Nascimento et al. HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA. PLoS One, v. 10, n. 9, p. 1-22, 2015.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/13545
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0139037
identifier_str_mv BRITO, Sieberth Nascimento et al. HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA. PLoS One, v. 10, n. 9, p. 1-22, 2015.
1932-6203
10.1371/journal.pone.0139037
url https://arca.fiocruz.br/handle/icict/13545
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://arca.fiocruz.br/bitstreams/209e0079-276a-4b30-b557-7b532c75810c/download
https://arca.fiocruz.br/bitstreams/18237924-053e-4add-a06d-d8ccc2cc2294/download
https://arca.fiocruz.br/bitstreams/0dcd0c7c-2b1d-4394-93ea-2c0bda0e3afb/download
https://arca.fiocruz.br/bitstreams/c67ac6b8-00a1-4950-a41f-69e2a1d51dec/download
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
ccf061d8ec236ad3a7bfe5a0f72915f2
6c96c116abbc06f78c53c2525b9432a1
75731f1b515ab69fc64e83b525adcac2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1853674431913656320
spelling 2016-04-05T18:20:47Z2016-04-05T18:20:47Z2015BRITO, Sieberth Nascimento et al. HIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA. PLoS One, v. 10, n. 9, p. 1-22, 2015.1932-6203https://arca.fiocruz.br/handle/icict/1354510.1371/journal.pone.0139037engPublic Library of ScienceHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Rio de Janeiro, RJ, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Grupo de Geno mica e Biologia Computacional. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório de Biocomplexidade e Genômica Evolutiva. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Informática em Saúde. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brasil.In order to establish new infections HIV-1 particles need to attach to receptors expressed on the cellular surface. HIV-1 particles interact with a cell membrane receptor known as CD4 and subsequently with another cell membrane molecule known as a co-receptor. Two major different co-receptors have been identified: C-C chemokine Receptor type 5 (CCR5) and C-X-C chemokine Receptor type 4 (CXCR4) Previous reports have demonstrated cellular modifications upon HIV-1 binding to its co-receptors including gene expression modulations. Here we investigated the effect of viral binding to either CCR5 or CXCR4 co-receptors on viral diversity after a single round of reverse transcription. CCR5 and CXCR4 pseudotyped viruses were used to infect non-stimulated and stimulated PBMCs and purified CD4 positive cells. We adopted the SOLiD methodology to sequence virtually the entire proviral DNA from all experimental infections. Infections with CCR5 and CXCR4 pseudotyped virus resulted in different patterns of genetic diversification. CCR5 virus infections produced extensive proviral diversity while in CXCR4 infections a more localized substitution process was observed. In addition, we present pioneering results of a recently developed method for the analysis of SOLiD generated sequencing data applicable to the study of viral quasi-species. Our findings demonstrate the feasibility of viral quasi-species evaluation by NGS methodologies. We presented for the first time strong evidence for a host cell driving mechanism acting on the HIV-1 genetic variability under the control of co-receptor stimulation. Additional investigations are needed to further clarify this question, which is relevant to viral diversification process and consequent disease progression.HIV-1Nucleotide sequencingCoreceptorsAmino acid substitutionReverse transcriptionSubstitution mutationViral genomicsHost cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZBrito, Sieberth NascimentoZukurov, Jean Paulo LopesMaricato, Juliana TerziVolpini, Angela CristinaSalim, Anna Christina de MatosAraújo, Flávio Marcos GomesCoimbra, Roney SantosOliveira, Guilherme Corrêa deAntoneli, FernandoJanini, Luiz Mário RamosLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/209e0079-276a-4b30-b557-7b532c75810c/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdfHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdfapplication/pdf4609751https://arca.fiocruz.br/bitstreams/18237924-053e-4add-a06d-d8ccc2cc2294/downloadccf061d8ec236ad3a7bfe5a0f72915f2MD52trueAnonymousREADTEXTHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdf.txtHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdf.txtExtracted texttext/plain1272https://arca.fiocruz.br/bitstreams/0dcd0c7c-2b1d-4394-93ea-2c0bda0e3afb/download6c96c116abbc06f78c53c2525b9432a1MD57falseAnonymousREADTHUMBNAILHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdf.jpgHIV-1 Tropism Determines Different Mutation Profiles in Proviral DNA..pdf.jpgGenerated Thumbnailimage/jpeg38085https://arca.fiocruz.br/bitstreams/c67ac6b8-00a1-4950-a41f-69e2a1d51dec/download75731f1b515ab69fc64e83b525adcac2MD58falseAnonymousREADicict/135452025-12-10 14:00:42.052open.accessoai:arca.fiocruz.br:icict/13545https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T17:00:42Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
score 15,300724

Similares en LA Referencia