Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth

Lectins have been used at length for basic research and clinical applications. New insights into the molecular recognition properties enhance our basic understanding of carbohydrate-protein interactions and aid in the design/development of new lectins. In this study, we used a combination of cell-ba...

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Bibliographic Details
Authors: Lubkowski, Jacek, Durbin, Sarah V., Silva, Mariana C. C. [UNIFESP], Farnsworth, David, Gildersleeve, Jeffrey C., Oliva, Maria Luiza V. [UNIFESP], Wlodawer, Alexander
Format: article
Status:Published version
Publication Date:2017
Country:Brasil
Institution:Universidade Federal de São Paulo (UNIFESP)
Repository:Repositório Institucional da UNIFESP
Language:English
OAI Identifier:oai:repositorio.unifesp.br:11600/55199
Online Access:http://dx.doi.org/10.1111/febs.13989
https://repositorio.unifesp.br/handle/11600/55199
Access Level:Open access
Keyword:cancer cell growth inhibition
carbohydrate binding
crystal structure
lectin
Tn antigen
Description
Summary:Lectins have been used at length for basic research and clinical applications. New insights into the molecular recognition properties enhance our basic understanding of carbohydrate-protein interactions and aid in the design/development of new lectins. In this study, we used a combination of cell-based assays, glycan microarrays, and X-ray crystallography to evaluate the structure and function of the recombinant Bauhinia forficata lectin (BfL). The lectin was shown to be cytostatic for several cancer cell lines included in the NCI-60 panel