Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools

Triatominae are hematophagous insects involved in the transmission of Chagas disease. Among the 19 genera of the subfamily, those with the highest epidemiological importance regarding the dissemination of Trypanosoma cruzi are Panstrongylus, Rhodnius, and Triatoma. Of these three genera, Rhodnius pr...

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Autores: dos Santos Souza, Éder [UNESP], Fernandes, Richard Perosa [UNESP], Guedes, Wesley Nascimento [UNESP], dos Santos, Fábio Neves, Eberlin, Marcos Nogueira, Lopes, Norberto Peporine, Padovani, Victor Damasceno [UNESP], da Rosa, João Aristeu [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/198387
Acceso en línea:http://dx.doi.org/10.1007/s00216-019-02376-y
http://hdl.handle.net/11449/198387
Access Level:acceso abierto
Palabra clave:Chemometric tools
MALDI-TOF MS
Mass spectrometry
Rhodnius
Triatominae
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spelling Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric toolsChemometric toolsMALDI-TOF MSMass spectrometryRhodniusTriatominaeTriatominae are hematophagous insects involved in the transmission of Chagas disease. Among the 19 genera of the subfamily, those with the highest epidemiological importance regarding the dissemination of Trypanosoma cruzi are Panstrongylus, Rhodnius, and Triatoma. Of these three genera, Rhodnius presents the greatest difficulties for specific identification. Thus, there is a need to overcome the difficulties in identifying phenotypes of similar species of this genus. In the present study, the MALDI-TOF MS methodology was used to identify 12 Rhodnius species, among the 21 admitted. The MALDI-TOF MS methodology allowed specific characterization through the identification of peptides and proteins, starting from four different methods of extraction: (A) acetonitrile/formic acid (ACN/AF), (B) acetonitrile/trifluoroacetic acid (ACN/TFA), (C) isopropyl/formic acid (IPA/AF), and (D) methanol/formic acid (MeOH/AF), and four types of MALDI-TOF matrices: α-cyano-4-hydroxycinnamic acid (CHCA), sinapic acid (SA), 6-aza-2-thiothymine (ATT), and 2,6-dihydroxyacetophenone (DHAP). The experiments were performed by combining the four solvents and four matrices to select the best MALDI extraction/matrix. The application of the MALDI-TOF MS technique, through the digital mass spectrometry approach combined with chemometric tools, such as partial least squares-discriminant analysis (PLS-DA), was able to discriminate 12 species of Rhodnius genus, which are difficult to identify using morphological characteristics. Thus, in view of the results obtained, the methodology described in the present article can be applied with speed and efficiency for the discrimination of Triatominae species. [Figure not available: see fulltext.]School of Pharmaceutical Sciences São Paulo State University (Unesp), Rodovia Araraquara-Jaú, km 1Institute of Chemistry São Paulo State University (Unesp)ThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas, CampinasDepartment of Chemistry and Physics School of Pharmaceutical Sciences of Ribeirão Preto São Paulo University, Av. do Café, s/nSchool of Pharmaceutical Sciences São Paulo State University (Unesp), Rodovia Araraquara-Jaú, km 1Institute of Chemistry São Paulo State University (Unesp)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)2020-12-12T01:11:27Z2020-12-12T01:11:27Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1431-1439http://dx.doi.org/10.1007/s00216-019-02376-yAnalytical and Bioanalytical Chemistry, v. 412, n. 6, p. 1431-1439, 2020.1618-26501618-2642http://hdl.handle.net/11449/19838710.1007/s00216-019-02376-y2-s2.0-85077696028Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytical and Bioanalytical Chemistryinfo:eu-repo/semantics/openAccessdos Santos Souza, Éder [UNESP]Fernandes, Richard Perosa [UNESP]Guedes, Wesley Nascimento [UNESP]dos Santos, Fábio NevesEberlin, Marcos NogueiraLopes, Norberto PeporinePadovani, Victor Damasceno [UNESP]da Rosa, João Aristeu [UNESP]2025-05-28T05:03:50Zoai:repositorio.unesp.br:11449/198387Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-28T05:03:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
title Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
spellingShingle Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
dos Santos Souza, Éder [UNESP]
Chemometric tools
MALDI-TOF MS
Mass spectrometry
Rhodnius
Triatominae
title_short Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
title_full Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
title_fullStr Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
title_full_unstemmed Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
title_sort Rhodnius spp. are differentiated based on the peptide/protein profile by matrix-assisted laser desorption/ionization mass spectrometry and chemometric tools
dc.creator.none.fl_str_mv dos Santos Souza, Éder [UNESP]
Fernandes, Richard Perosa [UNESP]
Guedes, Wesley Nascimento [UNESP]
dos Santos, Fábio Neves
Eberlin, Marcos Nogueira
Lopes, Norberto Peporine
Padovani, Victor Damasceno [UNESP]
da Rosa, João Aristeu [UNESP]
author dos Santos Souza, Éder [UNESP]
author_facet dos Santos Souza, Éder [UNESP]
Fernandes, Richard Perosa [UNESP]
Guedes, Wesley Nascimento [UNESP]
dos Santos, Fábio Neves
Eberlin, Marcos Nogueira
Lopes, Norberto Peporine
Padovani, Victor Damasceno [UNESP]
da Rosa, João Aristeu [UNESP]
author_role author
author2 Fernandes, Richard Perosa [UNESP]
Guedes, Wesley Nascimento [UNESP]
dos Santos, Fábio Neves
Eberlin, Marcos Nogueira
Lopes, Norberto Peporine
Padovani, Victor Damasceno [UNESP]
da Rosa, João Aristeu [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
dc.subject.por.fl_str_mv Chemometric tools
MALDI-TOF MS
Mass spectrometry
Rhodnius
Triatominae
topic Chemometric tools
MALDI-TOF MS
Mass spectrometry
Rhodnius
Triatominae
description Triatominae are hematophagous insects involved in the transmission of Chagas disease. Among the 19 genera of the subfamily, those with the highest epidemiological importance regarding the dissemination of Trypanosoma cruzi are Panstrongylus, Rhodnius, and Triatoma. Of these three genera, Rhodnius presents the greatest difficulties for specific identification. Thus, there is a need to overcome the difficulties in identifying phenotypes of similar species of this genus. In the present study, the MALDI-TOF MS methodology was used to identify 12 Rhodnius species, among the 21 admitted. The MALDI-TOF MS methodology allowed specific characterization through the identification of peptides and proteins, starting from four different methods of extraction: (A) acetonitrile/formic acid (ACN/AF), (B) acetonitrile/trifluoroacetic acid (ACN/TFA), (C) isopropyl/formic acid (IPA/AF), and (D) methanol/formic acid (MeOH/AF), and four types of MALDI-TOF matrices: α-cyano-4-hydroxycinnamic acid (CHCA), sinapic acid (SA), 6-aza-2-thiothymine (ATT), and 2,6-dihydroxyacetophenone (DHAP). The experiments were performed by combining the four solvents and four matrices to select the best MALDI extraction/matrix. The application of the MALDI-TOF MS technique, through the digital mass spectrometry approach combined with chemometric tools, such as partial least squares-discriminant analysis (PLS-DA), was able to discriminate 12 species of Rhodnius genus, which are difficult to identify using morphological characteristics. Thus, in view of the results obtained, the methodology described in the present article can be applied with speed and efficiency for the discrimination of Triatominae species. [Figure not available: see fulltext.]
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:11:27Z
2020-12-12T01:11:27Z
2020-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00216-019-02376-y
Analytical and Bioanalytical Chemistry, v. 412, n. 6, p. 1431-1439, 2020.
1618-2650
1618-2642
http://hdl.handle.net/11449/198387
10.1007/s00216-019-02376-y
2-s2.0-85077696028
url http://dx.doi.org/10.1007/s00216-019-02376-y
http://hdl.handle.net/11449/198387
identifier_str_mv Analytical and Bioanalytical Chemistry, v. 412, n. 6, p. 1431-1439, 2020.
1618-2650
1618-2642
10.1007/s00216-019-02376-y
2-s2.0-85077696028
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytical and Bioanalytical Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1431-1439
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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