A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal

Detection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-...

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Autores: Olean-Oliveira, André, Seraphim, Patrícia Monteiro [UNESP], Portugal, Miquéias L. [UNESP], Teixeira, Marcos F.S. [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/298662
Acesso em linha:http://dx.doi.org/10.1016/j.bios.2025.117219
https://hdl.handle.net/11449/298662
Access Level:acceso abierto
Palavra-chave:Anti-GLUT4
Electrochemical impedance spectroscopy
Glucose transporters
Gold nanoparticle polymer
Immunosensor
Physiological protocol
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spelling A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signalAnti-GLUT4Electrochemical impedance spectroscopyGlucose transportersGold nanoparticle polymerImmunosensorPhysiological protocolDetection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-sensitive response is mediated by a redox-conductive polymer based on thionine. The sensor platform was fabricated via a one-step electropolymerization of thionine and gold nanoparticles (AuNPs) onto a platinum screen-printed electrode (Olean-Oliveira et al., 2022a). The immunosensor was then constructed by physical adsorption of a GLUT4 antibody onto the poly(thionine)-AuNP composite surface. This label-free approach eliminates the need for secondary antibodies or enzymes. The immunosensor performance was evaluated using electrochemical impedance spectroscopy (EIS). The sensing mechanism relies on impedance changes; increasing GLUT4 concentrations lead to increased impedance due to enhanced surface blocking upon GLUT4-antibody binding. This interaction impedes oxygen diffusion to the polymer redox sites, resulting in increased electrical resistance. Analysis of the redox capacitance as a function of frequency demonstrates a decrease in the capacitive arc with increasing GLUT4 concentration.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Max-Planck Institute for Chemical Energy ConversionDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP), SPDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, SPDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP), SPDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, SPFAPESP: 2004/10130-0FAPESP: 2024/08769-6CNPq: 308232/2023-2CAPES: 88882.434480/2019–01Max-Planck Institute for Chemical Energy ConversionUniversidade Estadual Paulista (UNESP)2025-04-29T18:37:49Z2025-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bios.2025.117219Biosensors and Bioelectronics, v. 275.1873-42350956-5663https://hdl.handle.net/11449/29866210.1016/j.bios.2025.1172192-s2.0-85216845541Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiosensors and Bioelectronicsinfo:eu-repo/semantics/openAccessOlean-Oliveira, AndréSeraphim, Patrícia Monteiro [UNESP]Portugal, Miquéias L. [UNESP]Teixeira, Marcos F.S. [UNESP]2025-10-22T17:53:19Zoai:repositorio.unesp.br:11449/298662Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T17:53:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
title A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
spellingShingle A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
Olean-Oliveira, André
Anti-GLUT4
Electrochemical impedance spectroscopy
Glucose transporters
Gold nanoparticle polymer
Immunosensor
Physiological protocol
title_short A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
title_full A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
title_fullStr A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
title_full_unstemmed A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
title_sort A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal
dc.creator.none.fl_str_mv Olean-Oliveira, André
Seraphim, Patrícia Monteiro [UNESP]
Portugal, Miquéias L. [UNESP]
Teixeira, Marcos F.S. [UNESP]
author Olean-Oliveira, André
author_facet Olean-Oliveira, André
Seraphim, Patrícia Monteiro [UNESP]
Portugal, Miquéias L. [UNESP]
Teixeira, Marcos F.S. [UNESP]
author_role author
author2 Seraphim, Patrícia Monteiro [UNESP]
Portugal, Miquéias L. [UNESP]
Teixeira, Marcos F.S. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Max-Planck Institute for Chemical Energy Conversion
Universidade Estadual Paulista (UNESP)
dc.subject.por.fl_str_mv Anti-GLUT4
Electrochemical impedance spectroscopy
Glucose transporters
Gold nanoparticle polymer
Immunosensor
Physiological protocol
topic Anti-GLUT4
Electrochemical impedance spectroscopy
Glucose transporters
Gold nanoparticle polymer
Immunosensor
Physiological protocol
description Detection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-sensitive response is mediated by a redox-conductive polymer based on thionine. The sensor platform was fabricated via a one-step electropolymerization of thionine and gold nanoparticles (AuNPs) onto a platinum screen-printed electrode (Olean-Oliveira et al., 2022a). The immunosensor was then constructed by physical adsorption of a GLUT4 antibody onto the poly(thionine)-AuNP composite surface. This label-free approach eliminates the need for secondary antibodies or enzymes. The immunosensor performance was evaluated using electrochemical impedance spectroscopy (EIS). The sensing mechanism relies on impedance changes; increasing GLUT4 concentrations lead to increased impedance due to enhanced surface blocking upon GLUT4-antibody binding. This interaction impedes oxygen diffusion to the polymer redox sites, resulting in increased electrical resistance. Analysis of the redox capacitance as a function of frequency demonstrates a decrease in the capacitive arc with increasing GLUT4 concentration.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-29T18:37:49Z
2025-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bios.2025.117219
Biosensors and Bioelectronics, v. 275.
1873-4235
0956-5663
https://hdl.handle.net/11449/298662
10.1016/j.bios.2025.117219
2-s2.0-85216845541
url http://dx.doi.org/10.1016/j.bios.2025.117219
https://hdl.handle.net/11449/298662
identifier_str_mv Biosensors and Bioelectronics, v. 275.
1873-4235
0956-5663
10.1016/j.bios.2025.117219
2-s2.0-85216845541
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biosensors and Bioelectronics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,301603

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