The influence of splenic tissue on the survival and tcd4 and tcd8 lymphocyte rates in rats subjected to fecal peritonitis induction

Purpose: Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes. Methods: Thirty male Wistar rats were distributed...

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Bibliographic Details
Authors: Andrea Saade Daher Borjaili, Danilo Nagib Salomão Paulo, Tarcizo Afonso Nunes, Giseli Celestino Nunes, Julia Belizário Silveira, Flávia Heiderich Dall'orto, Gabriel Souza Lorenzoni, Matheus Eduardo Daher Borjaili, Bianca Prandi Campagnaro, Maressa Cristiane Malini de Lima, Marcela Souza Lima Paulo
Format: article
Status:Published version
Publication Date:2020
Country:Brasil
Institution:Universidade Federal de Minas Gerais (UFMG)
Repository:Repositório Institucional da UFMG
Language:English
OAI Identifier:oai:repositorio.ufmg.br:1843/63156
Online Access:https://doi.org/10.1590/s0102-865020200100000003
http://hdl.handle.net/1843/63156
Access Level:Open access
Keyword:Splenectomy
Spleen
Peritonitis
Immunophenotyping
Survival Rate
Rats
Description
Summary:Purpose: Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes. Methods: Thirty male Wistar rats were distributed into 3 groups of ten: spleen manipulation (SM); total splenectomy (TS); subtotal splenectomy with preservation of the inferior pole (IP). Rats were subjected to surgical procedure depending on the group. Seven days after surgery they underwent induction of peritonitis and survival time was recorded. All animals were subjected to two blood collections: before surgery and 70 days after it for TCD4/TCD8 lymphocyte counting. Results: Mean survival time was longer in the IP and SM groups and shorter in the TS group; there was significant difference between them. The comparison of the median number of CD4 did not present changes, whereas the comparison of the median number of CD8 decreased in the SM and IP groups. The correlation between the median number of TCD4 and TCD8 lymphocytes and the animals’ survival was not significant. Conclusion: The maintenance of splenic tissue contributed to increase the survival of rats and there was a change in the number of TCD8 lymphocytes

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