Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis

Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory pa...

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Detalhes bibliográficos
Autores: Costa, Victor [UNESP], El-Achkar, Vivian Narana [UNESP], de Barros, Patrícia Pimentel [UNESP], León, Jorge Esquiche, Ribeiro-Silva, Alfredo, Carlos, Román, Pignatari, Shirley Shizue Nagata, Ferreira, Silvaneide, Mello, Barbara Pereira, Sichero, Laura, Villa, Luisa Lina, Kaminagakura, Estela [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/199858
Acesso em linha:http://dx.doi.org/10.1002/lary.28465
http://hdl.handle.net/11449/199858
Access Level:acceso abierto
Palavra-chave:CD21
Epstein-Barr virus
human papilloma virus
severity
Squamous papillomatosis
Descrição
Resumo:Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.

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