Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis

The construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-lik...

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Detalles Bibliográficos
Autores: Costa, Carolina S. [UNESP], Bravo, Juliana P. [UNESP], Ribeiro, Cíntia L. [UNESP], Soprano, Adriana S. [UNESP], Sassaki, Flávio T. [UNESP], Maia, Ivan G. [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/179026
Acceso en línea:http://dx.doi.org/10.1007/s11240-017-1276-6
http://hdl.handle.net/11449/179026
Access Level:acceso abierto
Palabra clave:Eucalyptus
Potassium transporter
Promoter
Root
Vascular expression
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spelling Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandisEucalyptusPotassium transporterPromoterRootVascular expressionThe construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-like potassium (K+) transporter (designated EgHAK5) showing root-specific expression. The ability of the EgHAK 5′-flanking region (~1.3 kb) to drive root-specific expression of a reporter gene (β-glucuronidase; GUS) was examined using transgenic tobacco plants. Histochemical analysis revealed enhanced GUS staining in the vasculature of leaves, hypocotyls and roots, which was also confirmed in histological cross-sections. Moreover, the relative expression of GUS in the roots of the generated transgenic lines was increased in response to K+ starvation. Overall, our results indicate that, in a heterologous system, the EgHAK5 promoter shows expression in vascular tissues, mainly within the phloem, and is up-regulated upon potassium deprivation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Genética Instituto de Biociências UNESPMonsanto Company, 700 Chesterfield Pkwy WInstituto de Genética e Bioquímica Universidade Federal de Uberlândia, Campus Monte Carmelo, Rodovia LMG 746, Km 1 - Bairro ArarasDepartamento de Genética Instituto de Biociências UNESPFAPESP: 03/00724-7CNPq: 476149/04-5CNPq: 558413/08Universidade Estadual Paulista (Unesp)Monsanto CompanyUniversidade Federal de Uberlândia (UFU)2018-12-11T17:33:13Z2018-12-11T17:33:13Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article213-222application/pdfhttp://dx.doi.org/10.1007/s11240-017-1276-6Plant Cell, Tissue and Organ Culture, v. 131, n. 2, p. 213-222, 2017.1573-50440167-6857http://hdl.handle.net/11449/17902610.1007/s11240-017-1276-62-s2.0-850237451352-s2.0-85023745135.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Cell, Tissue and Organ Culture0,8550,855info:eu-repo/semantics/openAccessCosta, Carolina S. [UNESP]Bravo, Juliana P. [UNESP]Ribeiro, Cíntia L. [UNESP]Soprano, Adriana S. [UNESP]Sassaki, Flávio T. [UNESP]Maia, Ivan G. [UNESP]2025-10-23T11:28:28Zoai:repositorio.unesp.br:11449/179026Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-23T11:28:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
title Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
spellingShingle Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
Costa, Carolina S. [UNESP]
Eucalyptus
Potassium transporter
Promoter
Root
Vascular expression
title_short Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
title_full Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
title_fullStr Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
title_full_unstemmed Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
title_sort Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
dc.creator.none.fl_str_mv Costa, Carolina S. [UNESP]
Bravo, Juliana P. [UNESP]
Ribeiro, Cíntia L. [UNESP]
Soprano, Adriana S. [UNESP]
Sassaki, Flávio T. [UNESP]
Maia, Ivan G. [UNESP]
author Costa, Carolina S. [UNESP]
author_facet Costa, Carolina S. [UNESP]
Bravo, Juliana P. [UNESP]
Ribeiro, Cíntia L. [UNESP]
Soprano, Adriana S. [UNESP]
Sassaki, Flávio T. [UNESP]
Maia, Ivan G. [UNESP]
author_role author
author2 Bravo, Juliana P. [UNESP]
Ribeiro, Cíntia L. [UNESP]
Soprano, Adriana S. [UNESP]
Sassaki, Flávio T. [UNESP]
Maia, Ivan G. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Monsanto Company
Universidade Federal de Uberlândia (UFU)
dc.subject.por.fl_str_mv Eucalyptus
Potassium transporter
Promoter
Root
Vascular expression
topic Eucalyptus
Potassium transporter
Promoter
Root
Vascular expression
description The construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-like potassium (K+) transporter (designated EgHAK5) showing root-specific expression. The ability of the EgHAK 5′-flanking region (~1.3 kb) to drive root-specific expression of a reporter gene (β-glucuronidase; GUS) was examined using transgenic tobacco plants. Histochemical analysis revealed enhanced GUS staining in the vasculature of leaves, hypocotyls and roots, which was also confirmed in histological cross-sections. Moreover, the relative expression of GUS in the roots of the generated transgenic lines was increased in response to K+ starvation. Overall, our results indicate that, in a heterologous system, the EgHAK5 promoter shows expression in vascular tissues, mainly within the phloem, and is up-regulated upon potassium deprivation.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
2018-12-11T17:33:13Z
2018-12-11T17:33:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11240-017-1276-6
Plant Cell, Tissue and Organ Culture, v. 131, n. 2, p. 213-222, 2017.
1573-5044
0167-6857
http://hdl.handle.net/11449/179026
10.1007/s11240-017-1276-6
2-s2.0-85023745135
2-s2.0-85023745135.pdf
url http://dx.doi.org/10.1007/s11240-017-1276-6
http://hdl.handle.net/11449/179026
identifier_str_mv Plant Cell, Tissue and Organ Culture, v. 131, n. 2, p. 213-222, 2017.
1573-5044
0167-6857
10.1007/s11240-017-1276-6
2-s2.0-85023745135
2-s2.0-85023745135.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plant Cell, Tissue and Organ Culture
0,855
0,855
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 213-222
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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