Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test
Dogs are considered the major domestic reservoir for human visceral leishmaniasis, a serious disease caused by the Leishmania infantum parasite. Diagnosis of canine visceral leishmaniasis (CVL) is critical for disease control, with several methods currently available. Among the serological tests, th...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/304709 |
| Acceso en línea: | http://dx.doi.org/10.3390/vetsci10100608 https://hdl.handle.net/11449/304709 |
| Access Level: | acceso abierto |
| Palabra clave: | canine visceral leishmaniasis DPP test Leishmania infantum |
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Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Testcanine visceral leishmaniasisDPP testLeishmania infantumDogs are considered the major domestic reservoir for human visceral leishmaniasis, a serious disease caused by the Leishmania infantum parasite. Diagnosis of canine visceral leishmaniasis (CVL) is critical for disease control, with several methods currently available. Among the serological tests, the DPP rapid test and the EIE-LVC, more commonly used in Brazil, are associated with variable sensitivity and specificity. Research with novel recombinant proteins such as the ELISA with the recombinant chimeric protein Q5 may therefore improve the CVL diagnosis. This study aimed to evaluate the true diagnostic potential of Q5 in an ELISA assay using a large number of CVL-suspected sera (406) with a previous positive diagnosis based on the rapid DPP test. Sera from the DPP-positive dogs, also assessed with the EIE-LVC test, were compared with sera from healthy dogs (n = 46) and used for ELISA tests using the recombinant Q5. The resulting data as well as the correlation with the clinical signs and the environmental characteristics of the animals were analyzed using Medal and GraphPad Prism 8.0. Overall, similar levels of lower sensitivity (67–68%) were seen for both the commercial EIE-LVC test and the Q5 ELISA when all assessed sera were considered, but a much greater sensitivity (92%) was seen for those samples from symptomatic dogs only. In contrast, many negative results were observed for the DPP-positive sera from asymptomatic dogs or those with no clinical information available. A selection of those sera were tested yet again in new ELISA assays using a second batch of the recombinant Q5, purified under milder denaturing conditions, as well as using another recombinant protein (Lci13). The results reveal a higher-than-expected incidence of likely false-positive results for DPP, reinforcing the need for other recombinant proteins, such as the chimeric Q5, to be investigated as possible alternatives to the currently used CVL diagnostic methods.Postgraduate Program in Health Sciences University of PernambucoAggeu Magalhães Institute—FiocruzDepartment of Clinic Surgery and Animal Reproduction College of Veterinary Medicine São Paulo State UniversityDepartment of Clinic Surgery and Animal Reproduction College of Veterinary Medicine São Paulo State UniversityUniversity of PernambucoAggeu Magalhães Institute—FiocruzUniversidade Estadual Paulista (UNESP)2025-04-29T19:35:45Z2023-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/vetsci10100608Veterinary Sciences, v. 10, n. 10, 2023.2306-7381https://hdl.handle.net/11449/30470910.3390/vetsci101006082-s2.0-85175296210Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Sciencesinfo:eu-repo/semantics/openAccessde Araújo Paz, Larissa Ferreirada Silva, Adalúciada Silva, Hemilly Rayanne FerreiraCavalcanti, Milena Paivade Lima, Valeria Marçal Felix [UNESP]da Cunha Beltrão, Maria Rosário OliveiraSilva, Maria Beatriz Araújode Melo Neto, Osvaldo PompílioMedeiros, Zulma MariaSantos, Wagner José Tenório dos2025-06-07T05:00:59Zoai:repositorio.unesp.br:11449/304709Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-06-07T05:00:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| title |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| spellingShingle |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test de Araújo Paz, Larissa Ferreira canine visceral leishmaniasis DPP test Leishmania infantum |
| title_short |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| title_full |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| title_fullStr |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| title_full_unstemmed |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| title_sort |
Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test |
| dc.creator.none.fl_str_mv |
de Araújo Paz, Larissa Ferreira da Silva, Adalúcia da Silva, Hemilly Rayanne Ferreira Cavalcanti, Milena Paiva de Lima, Valeria Marçal Felix [UNESP] da Cunha Beltrão, Maria Rosário Oliveira Silva, Maria Beatriz Araújo de Melo Neto, Osvaldo Pompílio Medeiros, Zulma Maria Santos, Wagner José Tenório dos |
| author |
de Araújo Paz, Larissa Ferreira |
| author_facet |
de Araújo Paz, Larissa Ferreira da Silva, Adalúcia da Silva, Hemilly Rayanne Ferreira Cavalcanti, Milena Paiva de Lima, Valeria Marçal Felix [UNESP] da Cunha Beltrão, Maria Rosário Oliveira Silva, Maria Beatriz Araújo de Melo Neto, Osvaldo Pompílio Medeiros, Zulma Maria Santos, Wagner José Tenório dos |
| author_role |
author |
| author2 |
da Silva, Adalúcia da Silva, Hemilly Rayanne Ferreira Cavalcanti, Milena Paiva de Lima, Valeria Marçal Felix [UNESP] da Cunha Beltrão, Maria Rosário Oliveira Silva, Maria Beatriz Araújo de Melo Neto, Osvaldo Pompílio Medeiros, Zulma Maria Santos, Wagner José Tenório dos |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
University of Pernambuco Aggeu Magalhães Institute—Fiocruz Universidade Estadual Paulista (UNESP) |
| dc.subject.por.fl_str_mv |
canine visceral leishmaniasis DPP test Leishmania infantum |
| topic |
canine visceral leishmaniasis DPP test Leishmania infantum |
| description |
Dogs are considered the major domestic reservoir for human visceral leishmaniasis, a serious disease caused by the Leishmania infantum parasite. Diagnosis of canine visceral leishmaniasis (CVL) is critical for disease control, with several methods currently available. Among the serological tests, the DPP rapid test and the EIE-LVC, more commonly used in Brazil, are associated with variable sensitivity and specificity. Research with novel recombinant proteins such as the ELISA with the recombinant chimeric protein Q5 may therefore improve the CVL diagnosis. This study aimed to evaluate the true diagnostic potential of Q5 in an ELISA assay using a large number of CVL-suspected sera (406) with a previous positive diagnosis based on the rapid DPP test. Sera from the DPP-positive dogs, also assessed with the EIE-LVC test, were compared with sera from healthy dogs (n = 46) and used for ELISA tests using the recombinant Q5. The resulting data as well as the correlation with the clinical signs and the environmental characteristics of the animals were analyzed using Medal and GraphPad Prism 8.0. Overall, similar levels of lower sensitivity (67–68%) were seen for both the commercial EIE-LVC test and the Q5 ELISA when all assessed sera were considered, but a much greater sensitivity (92%) was seen for those samples from symptomatic dogs only. In contrast, many negative results were observed for the DPP-positive sera from asymptomatic dogs or those with no clinical information available. A selection of those sera were tested yet again in new ELISA assays using a second batch of the recombinant Q5, purified under milder denaturing conditions, as well as using another recombinant protein (Lci13). The results reveal a higher-than-expected incidence of likely false-positive results for DPP, reinforcing the need for other recombinant proteins, such as the chimeric Q5, to be investigated as possible alternatives to the currently used CVL diagnostic methods. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-10-01 2025-04-29T19:35:45Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/vetsci10100608 Veterinary Sciences, v. 10, n. 10, 2023. 2306-7381 https://hdl.handle.net/11449/304709 10.3390/vetsci10100608 2-s2.0-85175296210 |
| url |
http://dx.doi.org/10.3390/vetsci10100608 https://hdl.handle.net/11449/304709 |
| identifier_str_mv |
Veterinary Sciences, v. 10, n. 10, 2023. 2306-7381 10.3390/vetsci10100608 2-s2.0-85175296210 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Veterinary Sciences |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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