Evaluation of methods for Ochratoxin A determination in green and roasted coffees
Different extraction solvents and cIeanup techniques for mycotoxins determination were evaluated for green and roasted coffee. Classical techniques such as partition and cIarification as well as solid phase extraction (silica, octadecylsilyl, cianopropyl and immunoaffinity columns) were tested. Only...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1999 |
| País: | Brasil |
| Institución: | Instituto Adolfo Lutz |
| Repositorio: | Revista do Instituto Adolfo Lutz (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/36698 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/RIAL/article/view/36698 |
| Access Level: | acceso abierto |
| Palabra clave: | Ochratoxin A coffee mycotoxins Ocratoxina A café micotoxinas |
| Sumario: | Different extraction solvents and cIeanup techniques for mycotoxins determination were evaluated for green and roasted coffee. Classical techniques such as partition and cIarification as well as solid phase extraction (silica, octadecylsilyl, cianopropyl and immunoaffinity columns) were tested. Only immunoaffinity columns were capable to effectively remove interferents frorn coffee extracts allowing ochratoxin A to be determined by HPLC and fluorescence detection at levels of 0,7 ng/g. Two commercial brands of immunoaffinity columns were evaluated for ochratoxin A recovery and repeated use. Average recovery for one brand was 97% and was above 70% on the fourth use. Average recovery for a second brand was 73% on the first use and below 30% on the second use. |
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