Sensitivity of ELISA anti-PGL-1 test with two derived synthetic antigens of PGL-1 of Mycobacterium leprae
The diagnosis of leprosy is mainly clinical, but the identification of the main antigen of Mycobacterium leprae cell wall, phenolic glycolipid 1 (PGL-1), contributed to studies about humoral immunity in leprosy. This experimental study evaluated the sensitivity of ELISA anti-PGL-1...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Brasil |
| Institución: | Instituto Evandro Chagas (IEC) |
| Repositorio: | Revista Pan-Amazônica de Saúde (RPAS) |
| Idioma: | portugués |
| OAI Identifier: | oai:revista.iec.gov.br:article/755 |
| Acceso en línea: | https://ojs.iec.gov.br/rpas/article/view/755 |
| Access Level: | acceso abierto |
| Palabra clave: | Leprosy Antigens Enzyme-Linked Immunosorbent Assay Diagnosis Hanseníase Antígenos Ensaio de Imunoadsorção Enzimática Diagnóstico Lepra Ensayo de Inmunoadsorción Enzimática |
| Sumario: | The diagnosis of leprosy is mainly clinical, but the identification of the main antigen of Mycobacterium leprae cell wall, phenolic glycolipid 1 (PGL-1), contributed to studies about humoral immunity in leprosy. This experimental study evaluated the sensitivity of ELISA anti-PGL-1 test using two semisynthetic glycolipids, disaccharide (ND-O-BSA) and trisaccharide (NT-P-BSA) as a potential tool for assisting the diagnosis of leprosy. The sensitivity/specificity to trisaccharide and disaccharide antigens was 97% and 98% and 83.3% respectively and 96.1% comparing patients with multibacillary leprosy (MB), consanguineous contacts (CCOS) and non-consanguineous (NCCOS). The trisaccharide antigen (NT-P-BSA) had a better development in the diagnosis with MB leprosy patients (p < 0.0001) compared to disaccharide antigen (ND-O-BSA), which was more suitable for the diagnosis of leprosy in paucibacillary patients - PB (PB x CCOS sensitivity = 80%). Besides that, the trisaccharide antigen was more suitable in the epidemiological surveillance CNCOS (X2 = 12,979, p = 0.0003). In endemic regions, the use of two antigens in the serology of leprosy is helpful in analyzing serum-epidemiological aspects that are important to the diagnosis and monitoring of leprosy. |
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