Isolation of potencially pathogenic free-living amoebas in hospital dust
OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care un...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | Brasil |
| Institución: | Universidade de São Paulo (USP) |
| Repositorio: | Revista de Saúde Pública |
| Idioma: | portugués |
| OAI Identifier: | oai:revistas.usp.br:article/31578 |
| Acceso en línea: | https://www.revistas.usp.br/rsp/article/view/31578 |
| Access Level: | acceso abierto |
| Palabra clave: | Acanthamoeba^i1^sisolame Naegleria^i1^sisolame Hospitais Amoeba Poeira^i1^sanál Infecção hospitalar^i1^spreven Naegleria Amebas de vida livre Acanthamoeba^i2^sisolat Purification Naegleria^i2^sisolat Purification^i2^shospit Dust^i2^sanaly Cross infection Prevention control Free-living amoebas |
| Sumario: | OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room . The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera. |
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