Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae).
Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymor...
| Authors: | , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2013 |
| Country: | Brasil |
| Institution: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
| Repository: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
| Language: | English |
| OAI Identifier: | oai:www.alice.cnptia.embrapa.br:doc/978390 |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/978390 http://dx.doi.org/10.4238/2013.February.6.3 |
| Access Level: | Open access |
| Keyword: | Fruta tropical Cerrado Cagaita Marcador molecular Variação genética Genetic variation Microsatellite repeats |
| Summary: | Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10-4 and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species. |
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