The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli

The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The anal...

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Detalles Bibliográficos
Autores: Monteiro-Vitorello, Claudia B., Camargo, Luis E. A., Van Sluys, Marie A., Kitajima, João P., Truffi, Daniela, Do Amaral, Alexandre M., Harakava, Ricardo, De Oliveira, Julio C. F., Wood, Derek, De Oliveira, Mariana C., Miyaki, Cristina, Takita, Marco A., Da Silva, Ana C. R., Furlan, Luis R. [UNESP], Carraro, Dirce M., Camarotte, Giovana, Almeida Jr., Nalvo F., Carrer, Helaine, Coutinho, Luiz L., El-Dorry, Hamza A., Ferro, Maria I. T. [UNESP], Gagliardi, Paulo R., Giglioti, Eder, Goldman, Maria H. S., Goldman, Gustavo H., Kimura, Edna T., Ferro, Emer S., Kuramae, Eiko E. [UNESP], Lemos, Eliana G. M. [UNESP], Lemos, Manoel V. F. [UNESP], Mauro, Sonia M. Z. [UNESP], Machado, Marcos A., Marino, Celso L. [UNESP], Menck, Carlos F., Nunes, Luiz R., Oliveira, Regina C., Pereira, Gonçalo G., Siqueira, Walter, De Souza, Alessandra A., Tsai, Siu M., Zanca, A. S., Simpson, Andrew J. G., Brumbley, Stevens M., Setúbal, João C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/67815
Acceso en línea:http://dx.doi.org/10.1094/MPMI.2004.17.8.827
http://hdl.handle.net/11449/67815
Access Level:acceso abierto
Palabra clave:Actinomycetales
bacterial gene
bacterial genome
classification
DNA base composition
genetics
microbiology
molecular genetics
nucleotide sequence
pseudogene
sugarcane
Base Composition
Genes, Bacterial
Genome, Bacterial
Molecular Sequence Data
Pseudogenes
Saccharum
Actinobacteria (class)
Bacteria (microorganisms)
Leifsonia
Leifsonia xyli subsp. xyli
Posibacteria
Prokaryota
Saccharum hybrid cultivar
uncultured actinomycete
Descripción
Sumario:The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Although L. xyli subsp. xyli has only been identified colonizing the xylem vessels of sugarcane, the numbers of predicted regulatory genes and sugar transporters are similar to those in free-living organisms. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. The presence of the latter may contribute to stunting, since it is likely involved in the synthesis of abscisic acid, a hormone that arrests growth. Our findings are consistent with the nutritionally fastidious behavior exhibited by L. xyli subsp. xyli and suggest an ongoing adaptation to the restricted ecological niche it inhabits.

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