Lactobacillus reuteri DSM 17938 protects agains gastric damage induced by ethanol administration in mice: role of TRPV1/substance P axis

This study aimed to evaluate the effect of Lactobacillus reuteri DSM 17938 (DSM) on ethanol-induced gastric injury, and if its possible mechanism of action is related to inhibiting the transient receptor potential vanilloid type 1 (TRPV1). We evaluated the effect of supplementing 108 CFU•g body wt−1...

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Detalhes bibliográficos
Autores: Oliveira, Ana P., Souza, Luan K. M., Araújo, Thiago S. L., Araújo, Simone de, Nogueira, Kerolayne M., Sousa, Francisca Beatriz M., Silva, Renan O., Pacífico, Dvison M., Martins, Conceição S., Brito, Gerly Anne de C., Souza, Marcellus H.L.P., Medeiros, Jand Venes R.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:inglés
OAI Identifier:oai:repositorio.ufc.br:riufc/40262
Acesso em linha:http://www.repositorio.ufc.br/handle/riufc/40262
Access Level:acceso abierto
Palavra-chave:Gastrite
Gastritis
Etanol
Ethanol
Probióticos
Probiotics
Descrição
Resumo:This study aimed to evaluate the effect of Lactobacillus reuteri DSM 17938 (DSM) on ethanol-induced gastric injury, and if its possible mechanism of action is related to inhibiting the transient receptor potential vanilloid type 1 (TRPV1). We evaluated the effect of supplementing 108 CFU•g body wt−1•day−1 of DSM on ethanol-induced gastric injury. DSM significantly reduced the ulcer area (1.940 ± 1.121 mm2 ) with 3 days of pretreatment. The effects of DSM supplementation were reversed by Resiniferatoxin (RTX), TRPV1 agonist (3 nmol/kg p.o.). Substance P (SP) (1 µmol/L per 20 g) plus 50% ethanol resulted in hemorrhagic lesions, and DSM supplementation did not reverse the lesion area induced by administering SP. TRPV1 staining intensity was lower, SP, malondialdehyde (MDA) and nitrite levels were reduced, and restored normal levels of antioxidant parameters (glutathione and superoxide dismutase) in the gastric mucosa in mice treated with DSM. In conclusion, DSM exhibited gastroprotective activity through decreased expression of TRPV1 receptor and decreasing SP levels, with a consequent reduction of oxidative stress.

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