Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado

Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado. The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of...

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Detalles Bibliográficos
Autores: Mannise, Natalia, Trovati, R. Guilherme, Duarte, J. Mauricio B. [UNESP], Maldonado, Jesús E., González, Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/221012
Acceso en línea:http://dx.doi.org/10.32800/abc.2018.41.0315
http://hdl.handle.net/11449/221012
Access Level:acceso abierto
Palabra clave:Fecal DNA
Microsatellite loci
Neotropical canid
Real time PCR
ZFX–ZFY
Descripción
Sumario:Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado. The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.