Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV

This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in dec...

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Detalhes bibliográficos
Autores: Lima, Maria Fernanda Ferreira de, França, Gislainy Isrraiany da Silva, Souza, Diogo Jorge Fragoso de, Cabral, Analucia Guedes Silveira, Filho, Clayton Anderson de Azevedo, Lima, Ellison Neves de, Sousa, Carlos Eduardo Miranda de
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Federação das Indústrias do Estado do Paraná (FIEP)
Repositorio:Brazilian Journal of Health Review
Idioma:inglés
OAI Identifier:oai:ojs2.ojs.brazilianjournals.com.br:article/4053
Acesso em linha:https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053
Access Level:acceso abierto
Palavra-chave:Caffeine
Decaffeinated coffee
HPLC-UV
Validation
Descrição
Resumo:This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs.

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