Ochratoxin A determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography

Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficien...

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Detalhes bibliográficos
Autores: Prado,Guilherme, Oliveira,Marize Silva, Carvalho,Eliana Pinheiro, Lima,Luiz Carlos Oliveira, Veloso,Thais, Souza,Leandro Augusto Ferreira, Cardoso,Ana Cristina Ferreira
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:Brasil
Recursos:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
Repositorio:Food Science and Technology (Campinas)
Idioma:inglés
OAI Identifier:oai:scielo:S0101-20612003000400011
Acesso em linha:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000400011
Access Level:acceso abierto
Palavra-chave:ochratoxin A
beer
immunoaffinity
HPLC
Descrição
Resumo:Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.