Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat

Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of Augus...

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Autores: FARIA,Carla Bertechini, SANTOS,Fabiane Cristina dos, CASTRO,Fausto Fernandes de, SUTIL,Ariadne Ricieli, SERGIO,Luciana Marciano, SILVA,Milena Veronezi, MACHINSKI JUNIOR,Miguel, BARBOSA-TESSMANN,Ione Parra
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
Repositorio:Food Science and Technology (Campinas)
Idioma:inglés
OAI Identifier:oai:scielo:S0101-20612017000100103
Acceso en línea:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103
Access Level:acceso abierto
Palabra clave:Aspergillus flavus
aflatoxins
Bulgur wheat
PCR
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spelling Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheatAspergillus flavusaflatoxinsBulgur wheatPCRAbstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103Food Science and Technology v.37 n.1 2017reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/1678-457x.09316info:eu-repo/semantics/openAccessengFARIA,Carla BertechiniSANTOS,Fabiane Cristina dosCASTRO,Fausto Fernandes deSUTIL,Ariadne RicieliSERGIO,Luciana MarcianoSILVA,Milena VeroneziMACHINSKI JUNIOR,MiguelBARBOSA-TESSMANN,Ione Parra2017-02-21T00:00:00Zoai:scielo:S0101-20612017000100103Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2017-02-21T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
spellingShingle Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
FARIA,Carla Bertechini
Aspergillus flavus
aflatoxins
Bulgur wheat
PCR
title_short Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_full Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_fullStr Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_full_unstemmed Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_sort Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
dc.creator.none.fl_str_mv FARIA,Carla Bertechini
SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
author FARIA,Carla Bertechini
author_facet FARIA,Carla Bertechini
SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
author_role author
author2 SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
author2_role author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Aspergillus flavus
aflatoxins
Bulgur wheat
PCR
topic Aspergillus flavus
aflatoxins
Bulgur wheat
PCR
description Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-457x.09316
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.37 n.1 2017
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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