Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions
In this work, it was developed a novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a supermacroporous polyacrylamide cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate was optimized as a function of temperature (°C), substrate molar ratio (butyric...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/221150 |
| Acceso en línea: | http://dx.doi.org/10.24275/uam/izt/dcbi/revmexingquim/2018v17n1/Verissimo http://hdl.handle.net/11449/221150 |
| Access Level: | acceso abierto |
| Palabra clave: | Butyl butyrate Cryogel Entrapment Lipase Tripalmitin |
| Sumario: | In this work, it was developed a novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a supermacroporous polyacrylamide cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate was optimized as a function of temperature (°C), substrate molar ratio (butyric acid: n-butanol) and added water (% (v/v)). The bioreactor was also applied to interesterification reactions. The maximum esterification yield (46%) for butyl butyrate synthesis was found at 40 °C, with a butyric acid:n-butanol molar ratio of 1:1.43 and added water of 65% (v/v). The bioreactor showed to be reusable with four consecutive reuses in the esterification reaction, with a volumetric activity of 31.6 mmol/mL·h. An interesterification yield of 17.35% was achieved when the substrate concentrations of tripalmitin and triolein were both 15 mmol/L, 35% added water (v/v) and temperature of 40 °C. These results showed that the bioreactor is a promising biocatalyst for biotechnological applications. |
|---|