Estudo de superfícies inteligentes para desenvolvimento de nanobiossensores

The use of herbicides has increased considerably in recent years and as a consequence, it has also been an increased exposure of ecosystems and human life to these highly toxic compounds. The study and development of metolodologies with greater sensitivity, which aims at the detection of these pesti...

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Detalles Bibliográficos
Autor: Silva, Aline Cristine Nanuh da
Tipo de recurso: tesis de maestría
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal de São Carlos (UFSCAR)
Repositorio:Repositório Institucional da UFSCAR
Idioma:portugués
OAI Identifier:oai:repositorio.ufscar.br:20.500.14289/1179
Acceso en línea:https://repositorio.ufscar.br/handle/20.500.14289/1179
Access Level:acceso abierto
Palabra clave:biossensor
nanotecnologia
herbicidas
enzimas
nanobiossensores
nanociência
sensores
enzymes
herbicides
nanoscience
sensors
OUTROS
Descripción
Sumario:The use of herbicides has increased considerably in recent years and as a consequence, it has also been an increased exposure of ecosystems and human life to these highly toxic compounds. The study and development of metolodologies with greater sensitivity, which aims at the detection of these pesticides, are becoming extremely relevant. Thus, this study aimed to study the development of a nanobiosensor applying functionalized AFM tips and Atomic Force Spectroscopy (AFS) for detection of herbicides imazaquin and metsulfuronmethyl, ALS inhibitors. We studied the force and work of adhesion between AFM tips, unmodified and chemically modified by immobilization of ALS, and substrates contaminated with imazaquin and metsulfuron-methyl. In addition, the tips were characterized by infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). It was possible to see differences between the tips functionalized and not functionalized, analyze the properties of each individual and develop a nanobiosensor for detection of herbicides.