Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese
This study evaluated the microbiological quality of milk and Coalho cheese, the prevalence of enterotoxin genes, antimicrobial resistance and determined an inducible MLSB resistance phenotype by the D-test in strains of Staphylococcus aureus isolated from these products. Seventy samples of milk and...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Universidade Estadual de Londrina (UEL) |
| Repositorio: | Semina. Ciências Agrárias (Online) |
| Idioma: | inglés |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/31787 |
| Acceso en línea: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31787 |
| Access Level: | acceso abierto |
| Palabra clave: | Antimicrobial resistance Dairy products Coalho cheese Staphylococcal enterotoxins genes Staphylococcus aureus. Resistência antimicrobiana Produtos lácteos Queijo de coalho Genes de enterotoxinas estafilocócicas |
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oai:ojs.pkp.sfu.ca:article/31787 |
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BR |
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Brasil |
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| dc.title.none.fl_str_mv |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese Qualidade microbiológica, detecção de genes de enterotoxinas e resistência antimicrobiana de Staphylococcus aureus isolados de leite e queijo de Coalho |
| title |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| spellingShingle |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese Pereira, Cecília Teresa Muniz Antimicrobial resistance Dairy products Coalho cheese Staphylococcal enterotoxins genes Staphylococcus aureus. Resistência antimicrobiana Produtos lácteos Queijo de coalho Genes de enterotoxinas estafilocócicas Staphylococcus aureus. |
| title_short |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| title_full |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| title_fullStr |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| title_full_unstemmed |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| title_sort |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese |
| dc.creator.none.fl_str_mv |
Pereira, Cecília Teresa Muniz Oliveira, Diêgo Sávio Vasconcelos de Veloso, Vanessa Solano Silva, Sabina dos Santos Paulino Santos, Leidiane Sousa Lima Neto, Anísio Ferreira Oliveira, Felipe Araújo de Alcantara Melo, Maria Celeste Nunes de Soares, Maria José dos Santos |
| author |
Pereira, Cecília Teresa Muniz |
| author_facet |
Pereira, Cecília Teresa Muniz Oliveira, Diêgo Sávio Vasconcelos de Veloso, Vanessa Solano Silva, Sabina dos Santos Paulino Santos, Leidiane Sousa Lima Neto, Anísio Ferreira Oliveira, Felipe Araújo de Alcantara Melo, Maria Celeste Nunes de Soares, Maria José dos Santos |
| author_role |
author |
| author2 |
Oliveira, Diêgo Sávio Vasconcelos de Veloso, Vanessa Solano Silva, Sabina dos Santos Paulino Santos, Leidiane Sousa Lima Neto, Anísio Ferreira Oliveira, Felipe Araújo de Alcantara Melo, Maria Celeste Nunes de Soares, Maria José dos Santos |
| author2_role |
author author author author author author author author |
| dc.subject.por.fl_str_mv |
Antimicrobial resistance Dairy products Coalho cheese Staphylococcal enterotoxins genes Staphylococcus aureus. Resistência antimicrobiana Produtos lácteos Queijo de coalho Genes de enterotoxinas estafilocócicas Staphylococcus aureus. |
| topic |
Antimicrobial resistance Dairy products Coalho cheese Staphylococcal enterotoxins genes Staphylococcus aureus. Resistência antimicrobiana Produtos lácteos Queijo de coalho Genes de enterotoxinas estafilocócicas Staphylococcus aureus. |
| description |
This study evaluated the microbiological quality of milk and Coalho cheese, the prevalence of enterotoxin genes, antimicrobial resistance and determined an inducible MLSB resistance phenotype by the D-test in strains of Staphylococcus aureus isolated from these products. Seventy samples of milk and Coalho cheese were analyzed. S. aureus strains were identified by biochemical tests. The presence of se genes (sea-see) was tested by polymerase chain reaction. The antimicrobial sensitivity of S. aureus strains was evaluated for 13 antimicrobial drugs using the disk diffusion technique and the double-disk diffusion test (D-test) was performed to determine inducible resistance to lincosamide phenotype. The amount of toxin sufficient to cause foodborne diseases is generally observed when Staphylococcus populations exceed 105 CFU mL-1 g-1. In this study, none of the milk samples analyzed showed these counts; however, 73.3% (22/30) of Coalho cheese samples exceeded this value. A total of 109 isolates were identified as S. aureus. The presence of enterotoxin genes was detected in 25.7% of these isolates and amplified only for the sec gene. Most of the isolates (78.5%) were resistant to one or more antimicrobial agents. The D test showed that 25.0% of erythromycin-resistant isolates had the constitutive resistance phenotype, and 3.8% had the inducible resistance phenotype to clindamycin. These results indicate that these dairy products represent a health risk since these bacteria can cause foodborne diseases or may be a possible route for the transfer of antimicrobial resistance to humans. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-08-20 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Descriptive study Estudo descritivo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31787 10.5433/1679-0359.2018v39n5p1957 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31787 |
| identifier_str_mv |
10.5433/1679-0359.2018v39n5p1957 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31787/24313 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
UEL |
| publisher.none.fl_str_mv |
UEL |
| dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 1957-1968 Semina: Ciências Agrárias; v. 39 n. 5 (2018); 1957-1968 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
| instname_str |
Universidade Estadual de Londrina (UEL) |
| instacron_str |
UEL |
| institution |
UEL |
| reponame_str |
Semina. Ciências Agrárias (Online) |
| collection |
Semina. Ciências Agrárias (Online) |
| repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
| repository.mail.fl_str_mv |
semina.agrarias@uel.br |
| _version_ |
1853676497620959232 |
| spelling |
Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheeseQualidade microbiológica, detecção de genes de enterotoxinas e resistência antimicrobiana de Staphylococcus aureus isolados de leite e queijo de CoalhoAntimicrobial resistanceDairy productsCoalho cheeseStaphylococcal enterotoxins genesStaphylococcus aureus.Resistência antimicrobianaProdutos lácteosQueijo de coalhoGenes de enterotoxinas estafilocócicasStaphylococcus aureus.This study evaluated the microbiological quality of milk and Coalho cheese, the prevalence of enterotoxin genes, antimicrobial resistance and determined an inducible MLSB resistance phenotype by the D-test in strains of Staphylococcus aureus isolated from these products. Seventy samples of milk and Coalho cheese were analyzed. S. aureus strains were identified by biochemical tests. The presence of se genes (sea-see) was tested by polymerase chain reaction. The antimicrobial sensitivity of S. aureus strains was evaluated for 13 antimicrobial drugs using the disk diffusion technique and the double-disk diffusion test (D-test) was performed to determine inducible resistance to lincosamide phenotype. The amount of toxin sufficient to cause foodborne diseases is generally observed when Staphylococcus populations exceed 105 CFU mL-1 g-1. In this study, none of the milk samples analyzed showed these counts; however, 73.3% (22/30) of Coalho cheese samples exceeded this value. A total of 109 isolates were identified as S. aureus. The presence of enterotoxin genes was detected in 25.7% of these isolates and amplified only for the sec gene. Most of the isolates (78.5%) were resistant to one or more antimicrobial agents. The D test showed that 25.0% of erythromycin-resistant isolates had the constitutive resistance phenotype, and 3.8% had the inducible resistance phenotype to clindamycin. These results indicate that these dairy products represent a health risk since these bacteria can cause foodborne diseases or may be a possible route for the transfer of antimicrobial resistance to humans.Este estudo avaliou a qualidade microbiológica de amostras de leite e queijo de Coalho, a prevalência de genes das enterotoxinas, a resistência antimicrobiana e o fenótipo de resistência MLSB por meio do D-teste, em cepas de Staphylococcus aureus isoladas destes produtos. Foram analisadas 70 amostras de leite e queijo de coalho. Cepas de S. aureus foram identificadas por meio de testes bioquímicos. A presença de genes se (sea-see) foi verificada pela técnica da reação em cadeia da polimerase (PCR). A sensibilidade antimicrobiana das cepas de S. aureus foi avaliada para 13 antimicrobianos, utilizando a técnica de difusão em disco e o ensaio de difusão de disco duplo (D-teste), para determinar o fenótipo de resistência induzível à lincosamidas. A quantidade de enterotoxina estafilocócica suficiente para causar a intoxicação alimentar é geralmente observada quando a população de estafilococos excede a contagem de 105 UFC mL-1 g-1. Neste estudo, nenhuma das amostras de leite analisadas apresentou esta contagem, entretanto, 73,3 % (22/30) das amostras de queijo de Coalho ultrapassaram este valor. Um total de 109 isolados foram identificados como S. aureus. A presença de genes de enterotoxinas foi detectada em 25,7% destas estirpes, ocorrendo amplificação apenas para o gene sec. A maioria destas bactérias (78,5 %) apresentaram resistência a um ou mais de dois agentes antimicrobianos. O D-teste demostrou que 25,0 % dos isolados resistentes à eritromicina tinham o fenótipo de resistência constitutiva e 3,8 % tinham o fenótipo de resistência induzível à clindamicina. Estes resultados indicam que estes produtos lácteos representam um risco para a saúde, desde que essas bactérias podem causar doenças de origem alimentar ou podem ser um possível caminho para a transferência de cepas resistentes aos antimicrobianos para os seres humanos.UEL2018-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionDescriptive studyEstudo descritivoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3178710.5433/1679-0359.2018v39n5p1957Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 1957-1968Semina: Ciências Agrárias; v. 39 n. 5 (2018); 1957-19681679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31787/24313Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPereira, Cecília Teresa MunizOliveira, Diêgo Sávio Vasconcelos deVeloso, Vanessa SolanoSilva, Sabina dos Santos PaulinoSantos, Leidiane SousaLima Neto, Anísio FerreiraOliveira, Felipe Araújo de AlcantaraMelo, Maria Celeste Nunes deSoares, Maria José dos Santos2022-10-20T17:48:50Zoai:ojs.pkp.sfu.ca:article/31787Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-20T17:48:50Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
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15,300719 |