Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLI...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | Instituto Nacional de Pesquisas da Amazônia (INPA) |
| Repositorio: | Repositório Institucional do INPA |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio:1/15017 |
| Acceso en línea: | https://repositorio.inpa.gov.br/handle/1/15017 |
| Access Level: | acceso abierto |
| Palabra clave: | Biotin Digoxigenin Algorithm Chromosome Color Controlled Study In Situ Hybridization, Fluorescence Image Analysis Quantitative Analysis Evaluation Study Microscopy, Fluorescence Image Processing Nucleic Acid Hybridization Procedures Software Chromosomes Evaluation Studies As Topic Image Processing, Computer-assisted |
| Sumario: | The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors. |
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