Fertilidade de espermatozóides recuperados da cauda do epidídimo de garanhões subférteis
Epididymal sperm harvest aims the recovery of genetic material from valuable stallions that gone dead, euthanized or with obstructive processes. However, subfertile stallions may also benefit from this technique, since epididymal sperm has no contact with potentially deleterious seminal plasma (SP)....
| Autores: | , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/141272 |
| Acesso em linha: | http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/93 http://hdl.handle.net/11449/141272 |
| Access Level: | acceso abierto |
| Palavra-chave: | Stallion Semen Epididymis Cryopreservation Technology Semental Epidídimo Criopreservación Biotecnologia Garanhão Sêmen Criopreservação |
| Resumo: | Epididymal sperm harvest aims the recovery of genetic material from valuable stallions that gone dead, euthanized or with obstructive processes. However, subfertile stallions may also benefit from this technique, since epididymal sperm has no contact with potentially deleterious seminal plasma (SP). The goal of the present study was to compare sperm viability between ejaculated (Group 1, G1) and epididymal cauda sperm (Group 2, G2) and to test fertility of epididymal sperm post-thaw from subfertile stallions. Two stallions from Mangalarga Marchador breed with history of subfertility were used. Three ejaculates from each stallion were collected and frozen in G1. One week later the stallions were castrated and sperm were harvested from epididymis using retrograde flushing (G2) with Botu-SêmenTM and then frozen with Botu-CrioTM. For fertility trial, 8 mares were inseminated with postthawed epididymal sperm from G2. Immediately after the detection of ovulation, mares were inseminated with 800x106 sperm (400 x106 from each stallion) in tip of uterine horn. All sperm parameters were analyzed by ANOVA followed by Tukey’s test to identify significant differences (p<0.05). Sperm concentration obtained in G1 and G2 were, respectively: 6.9±3.37b e 20.4±5.37a x 109 . Sperm parameters for G1 and G2 before freezing were, respectively: a) Total Motility (TM): 16.0±6.16b ; 66.5±6.24a ; b) Progressive Motility (PM): 4.5±2.52b ; 26.3±7.93ª; c) Rapid Sperm (RAP): 7.7±4.27b ; 48.8±5.38a ; d) Plasma Membrane Integrity (PMI): 26.8±12.36a ; 46.3±19.87ª; frozen-thawed parameters were: a)TM: 7.7±2.22b ; 33.3±2.06ª; b) PM:1.8±1.26b ; 12.5±3.32ª; c)RAP: 2±1.41b ; 19.3±1.26ª; d)PMI: 22.3±6.02b ; 41.8±10.59ª. Sperm parameters (TM and RAP) before and after freezing were superior in G2. Sperm harvested from epididymal cauda were not in contact with SP, which can be one of the possible causes of G1 increase on sperm viability. The conception rate obtained in G2 was 25% (2/8). Based on these results, we can conclude that harvesting sperm from epididymal cauda of stallions with poor semen viability has increased on TM and RAP when compared to the ejaculate. Moreover, pregnancy rates of 25% demonstrate that post-thawed epididymal cauda sperm were able to fertilize the oocyte. This result demonstrates the possibility to obtain foals from epididymal cauda sperm of subfertile stallions. However, more studies are necessary to identify the possible detrimental effects of SP on semen quality of infertile and subfertile stallions. |
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