Edaravone inhibits the production of reactive oxygen species in phagocytosis- and pkc-stimulated granulocytes from multiple sclerosis patients edaravone modulate oxidative stress in multiple sclerosis
BACKGROUND: Oxidative stress is associated with the pathogenesis of MS. Edaravone (EDV) has been proposed as a therapeutic resource for central nervous system diseases, and it was effective in reducing oxidative stress. However, the antioxidant mechanisms of EDV are poorly studied. OBJECTIVE: This s...
| Autores: | , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2022 |
| País: | Brasil |
| Recursos: | Universidade Federal de Minas Gerais (UFMG) |
| Repositório: | Repositório Institucional da UFMG |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/61856 |
| Acesso em linha: | https://doi.org/10.1177/11795735221092524 http://hdl.handle.net/1843/61856 https://orcid.org/0000-0003-1224-5243 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Multiple Sclerosis Edaravone Oxidative stress modulation Esclerose Múltipla Estresse Oxidativo |
| Resumo: | BACKGROUND: Oxidative stress is associated with the pathogenesis of MS. Edaravone (EDV) has been proposed as a therapeutic resource for central nervous system diseases, and it was effective in reducing oxidative stress. However, the antioxidant mechanisms of EDV are poorly studied. OBJECTIVE: This study aimed to evaluate the effects of EDV on resting, phagocytosis, and PKC-activated granulocytes derived from MS patients and a healthy control group. METHODS: The effects of EDV on ROS production in phagocytosis (ROS production in the presence of opsonized particles) and PKC-stimulated granulocytes were evaluated in a luminol-dependent chemiluminescence method. Calphostin C was used in some experiments to compare with those of EDV. RESULTS: EDV inhibited ROS production in phagocytosis of opsonized particles and PKC-stimulated granulocytes from MS patients and healthy control group. In the presence of calphostin C, the inhibition of ROS production was similar to that observed with EDV. CONCLUSION: These findings suggest the involvement of EDV on the ROS-PKC-NOX signaling pathways modulating oxidative stress in MS. EDV represents a promising treatment option to control oxidative innate immune response for MS. KEYWORDS: edaravone, multiple sclerosis, innate immunity, reactive oxygen species, phagocytosis, protein kinase C. |
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