EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.

Euphorbia tirucalli, a plant commonly known as aveloz, has been employed in anticancer therapy with little scientific proof. The goal of this study was to evaluate the cytotoxic potential and expression of caspase-3 and p53 in colorectal cancer cell lines treated with aveloz extracts. Hexanic and hy...

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Authors: Archanjo, Anderson Barros, Careta, Francisco de Paula, Costa, Adilson Vidal, Nunes, Louisiane de Carvalho
Format: article
Status:Published version
Publication Date:2016
Country:Brasil
Institution:Universidade Federal do Paraná (UFPR)
Repository:Archives of Veterinary Science (Online)
Language:English
OAI Identifier:oai:ojs.pkp.sfu.ca:article/41901
Online Access:https://revistas.ufpr.br/veterinary/article/view/41901
Access Level:Open access
Keyword:anticancer therapy
apoptosis
cell culture
Euphorbiaceae
immunocytochemistry
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spelling EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.anticancer therapyapoptosiscell cultureEuphorbiaceaeimmunocytochemistryEuphorbia tirucalli, a plant commonly known as aveloz, has been employed in anticancer therapy with little scientific proof. The goal of this study was to evaluate the cytotoxic potential and expression of caspase-3 and p53 in colorectal cancer cell lines treated with aveloz extracts. Hexanic and hydroalcoholic extracts were prepared in different concentrations. After 96 h, cell viability and apoptosis rate (p53 and caspase-3 expression) were verified. The hexanic extract revealed higher cytotoxicity on HCT-116 cell line at concentrations above 125 µg/mL, and hydroalcoholic extract above 500 µg/mL. LC50 for the hexanic extract was 60.2873 µg/mL, and for hydroalcoholic extract 100.6066 µg/mL. There were significant differences among the averages of cells marked with p53 at the concentrations 62.5, 250 and 500 µg/mL, as well as among the averages of caspase-3-positive cells at the concentrations 62.5, 125 and 500 µg/mL, with higher cytotoxic effect being observed for the hydroalcoholic extract. The obtained data provides evidence for the cytotoxic potential in vitro of the hydroalcoholic and hexanic extracts of aveloz on HCT-116 cell line. There was an increase in the expression of caspase-3 and p53 in the tumor line cells exposed to extracts of E. tirucalli.Universidade Federal do Paraná2016-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufpr.br/veterinary/article/view/4190110.5380/avs.v21i4.41901Archives of Veterinary Science; v. 21 n. 4 (2016)Archives of Veterinary Science; Vol. 21 No. 4 (2016)2317-68221517-784X10.5380/avs.v21i4reponame:Archives of Veterinary Science (Online)instname:Universidade Federal do Paraná (UFPR)instacron:UFPRenghttps://revistas.ufpr.br/veterinary/article/view/41901/29935Copyright (c) 2016 Archives of Veterinary Scienceinfo:eu-repo/semantics/openAccessArchanjo, Anderson BarrosCareta, Francisco de PaulaCosta, Adilson VidalNunes, Louisiane de Carvalho2016-12-30T22:32:06Zoai:ojs.pkp.sfu.ca:article/41901Revistahttps://revistas.ufpr.br/veterinary/indexPUBhttps://revistas.ufpr.br/veterinary/oaieditor.avs@ufpr.br || publish.avs@ufpr.br2317-68221517-784Xopendoar:2016-12-30T22:32:06Archives of Veterinary Science (Online) - Universidade Federal do Paraná (UFPR)false
dc.title.none.fl_str_mv EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
title EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
spellingShingle EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
Archanjo, Anderson Barros
anticancer therapy
apoptosis
cell culture
Euphorbiaceae
immunocytochemistry
title_short EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
title_full EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
title_fullStr EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
title_full_unstemmed EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
title_sort EVALUATION OF CYTOTOXICITY AND EXPRESSION OF CASPASE-3 AND P53 IN HCT-116 CELLS OF LINEAGE TREATED WITH DIFFERENT EXTRACTS OF Euphorbia tirucalli L.
dc.creator.none.fl_str_mv Archanjo, Anderson Barros
Careta, Francisco de Paula
Costa, Adilson Vidal
Nunes, Louisiane de Carvalho
author Archanjo, Anderson Barros
author_facet Archanjo, Anderson Barros
Careta, Francisco de Paula
Costa, Adilson Vidal
Nunes, Louisiane de Carvalho
author_role author
author2 Careta, Francisco de Paula
Costa, Adilson Vidal
Nunes, Louisiane de Carvalho
author2_role author
author
author
dc.subject.por.fl_str_mv anticancer therapy
apoptosis
cell culture
Euphorbiaceae
immunocytochemistry
topic anticancer therapy
apoptosis
cell culture
Euphorbiaceae
immunocytochemistry
description Euphorbia tirucalli, a plant commonly known as aveloz, has been employed in anticancer therapy with little scientific proof. The goal of this study was to evaluate the cytotoxic potential and expression of caspase-3 and p53 in colorectal cancer cell lines treated with aveloz extracts. Hexanic and hydroalcoholic extracts were prepared in different concentrations. After 96 h, cell viability and apoptosis rate (p53 and caspase-3 expression) were verified. The hexanic extract revealed higher cytotoxicity on HCT-116 cell line at concentrations above 125 µg/mL, and hydroalcoholic extract above 500 µg/mL. LC50 for the hexanic extract was 60.2873 µg/mL, and for hydroalcoholic extract 100.6066 µg/mL. There were significant differences among the averages of cells marked with p53 at the concentrations 62.5, 250 and 500 µg/mL, as well as among the averages of caspase-3-positive cells at the concentrations 62.5, 125 and 500 µg/mL, with higher cytotoxic effect being observed for the hydroalcoholic extract. The obtained data provides evidence for the cytotoxic potential in vitro of the hydroalcoholic and hexanic extracts of aveloz on HCT-116 cell line. There was an increase in the expression of caspase-3 and p53 in the tumor line cells exposed to extracts of E. tirucalli.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufpr.br/veterinary/article/view/41901
10.5380/avs.v21i4.41901
url https://revistas.ufpr.br/veterinary/article/view/41901
identifier_str_mv 10.5380/avs.v21i4.41901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufpr.br/veterinary/article/view/41901/29935
dc.rights.driver.fl_str_mv Copyright (c) 2016 Archives of Veterinary Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Archives of Veterinary Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Paraná
publisher.none.fl_str_mv Universidade Federal do Paraná
dc.source.none.fl_str_mv Archives of Veterinary Science; v. 21 n. 4 (2016)
Archives of Veterinary Science; Vol. 21 No. 4 (2016)
2317-6822
1517-784X
10.5380/avs.v21i4
reponame:Archives of Veterinary Science (Online)
instname:Universidade Federal do Paraná (UFPR)
instacron:UFPR
instname_str Universidade Federal do Paraná (UFPR)
instacron_str UFPR
institution UFPR
reponame_str Archives of Veterinary Science (Online)
collection Archives of Veterinary Science (Online)
repository.name.fl_str_mv Archives of Veterinary Science (Online) - Universidade Federal do Paraná (UFPR)
repository.mail.fl_str_mv editor.avs@ufpr.br || publish.avs@ufpr.br
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score 15,300719

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