JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region

This study evaluated the relative occurrences of BK virus (BKV) and JC virus (JCV) infections in patients with chronic kidney disease (CKD). Urine samples were analysed from CKD patients and from 99 patients without CKD as a control. A total of 100 urine samples were analysed from the experimental (...

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Autores: MELO, Fernando de Assis Ferreira, BEZERRA, Ana Caroline Fonseca, SANTANA, Bárbara Brasil, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo, VALLINOTO, Izaura Maria Vieira Cayres, VALLINOTO, Antonio Carlos Rosário
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Recursos:Universidade Federal do Pará (UFPA)
Repositorio:Repositório Institucional da UFPA
Idioma:inglés
OAI Identifier:oai:repositorio.ufpa.br:2011/5712
Acesso em linha:http://repositorio.ufpa.br/jspui/handle/2011/5712
Access Level:acceso abierto
Palavra-chave:Insuficiência renal crônica
Infecções por polyomavirus
Transplante de rim
Vírus BK
Vírus JC
Amazônia brasileira
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repository_id_str
dc.title.pt_BR.fl_str_mv JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
title JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
spellingShingle JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
MELO, Fernando de Assis Ferreira
Insuficiência renal crônica
Infecções por polyomavirus
Transplante de rim
Vírus BK
Vírus JC
Amazônia brasileira
title_short JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
title_full JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
title_fullStr JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
title_full_unstemmed JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
title_sort JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region
dc.creator.none.fl_str_mv MELO, Fernando de Assis Ferreira
BEZERRA, Ana Caroline Fonseca
SANTANA, Bárbara Brasil
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Izaura Maria Vieira Cayres
VALLINOTO, Antonio Carlos Rosário
author MELO, Fernando de Assis Ferreira
author_facet MELO, Fernando de Assis Ferreira
BEZERRA, Ana Caroline Fonseca
SANTANA, Bárbara Brasil
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Izaura Maria Vieira Cayres
VALLINOTO, Antonio Carlos Rosário
author_role author
author2 BEZERRA, Ana Caroline Fonseca
SANTANA, Bárbara Brasil
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Izaura Maria Vieira Cayres
VALLINOTO, Antonio Carlos Rosário
author2_role author
author
author
author
author
author
dc.subject.por.fl_str_mv Insuficiência renal crônica
Infecções por polyomavirus
Transplante de rim
Vírus BK
Vírus JC
Amazônia brasileira
topic Insuficiência renal crônica
Infecções por polyomavirus
Transplante de rim
Vírus BK
Vírus JC
Amazônia brasileira
description This study evaluated the relative occurrences of BK virus (BKV) and JC virus (JCV) infections in patients with chronic kidney disease (CKD). Urine samples were analysed from CKD patients and from 99 patients without CKD as a control. A total of 100 urine samples were analysed from the experimental (CKD patients) group and 99 from the control group. Following DNA extraction, polymerase chain reaction (PCR) was used to amplify a 173 bp region of the gene encoding the T antigen of the BKV and JCV. JCV and BKV infections were differentiated based on the enzymatic digestion of the amplified products using BamHI endonuclease. The results indicated that none of the patients in either group was infected with the BKV, whereas 11.1% (11/99) of the control group subjects and 4% (4/100) of the kidney patients were infected with the JCV. High levels of urea in the excreted urine, low urinary cellularity, reduced bladder washout and a delay in analysing the samples may have contributed to the low prevalence of infection. The results indicate that there is a need to increase the sensitivity of assays used to detect viruses in patients with CDK, especially given that polyomavirus infections, especially BKV, can lead to a loss of kidney function following transplantation.
publishDate 2013
dc.date.issued.fl_str_mv 2013-04
dc.date.accessioned.fl_str_mv 2014-09-15T12:51:43Z
dc.date.available.fl_str_mv 2014-09-15T12:51:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MELO, Fernando Assis Ferreira et al. JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 2, p. 145-149, abr. 2013. Disponível em: <http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0145.pdf>. Acesso em: 18 jun. 2014. http://dx.doi.org/10.1590/0074-0276108022013003.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/5712
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv MELO, Fernando Assis Ferreira et al. JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 2, p. 145-149, abr. 2013. Disponível em: <http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0145.pdf>. Acesso em: 18 jun. 2014. http://dx.doi.org/10.1590/0074-0276108022013003.
0074-0276
url http://repositorio.ufpa.br/jspui/handle/2011/5712
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPA
instname:Universidade Federal do Pará (UFPA)
instacron:UFPA
instname_str Universidade Federal do Pará (UFPA)
instacron_str UFPA
institution UFPA
reponame_str Repositório Institucional da UFPA
collection Repositório Institucional da UFPA
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spelling 2014-09-15T12:51:43Z2014-09-15T12:51:43Z2013-04MELO, Fernando Assis Ferreira et al. JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon Region. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 2, p. 145-149, abr. 2013. Disponível em: <http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0145.pdf>. Acesso em: 18 jun. 2014. http://dx.doi.org/10.1590/0074-0276108022013003.0074-0276http://repositorio.ufpa.br/jspui/handle/2011/5712This study evaluated the relative occurrences of BK virus (BKV) and JC virus (JCV) infections in patients with chronic kidney disease (CKD). Urine samples were analysed from CKD patients and from 99 patients without CKD as a control. A total of 100 urine samples were analysed from the experimental (CKD patients) group and 99 from the control group. Following DNA extraction, polymerase chain reaction (PCR) was used to amplify a 173 bp region of the gene encoding the T antigen of the BKV and JCV. JCV and BKV infections were differentiated based on the enzymatic digestion of the amplified products using BamHI endonuclease. The results indicated that none of the patients in either group was infected with the BKV, whereas 11.1% (11/99) of the control group subjects and 4% (4/100) of the kidney patients were infected with the JCV. High levels of urea in the excreted urine, low urinary cellularity, reduced bladder washout and a delay in analysing the samples may have contributed to the low prevalence of infection. The results indicate that there is a need to increase the sensitivity of assays used to detect viruses in patients with CDK, especially given that polyomavirus infections, especially BKV, can lead to a loss of kidney function following transplantation.Submitted by Edisangela Bastos (edisangela@ufpa.br) on 2014-08-29T18:55:20Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Artigo_JcPolyomavirusInfection.pdf: 341246 bytes, checksum: 9154f8afe5e766dbe7f3364adf29e89d (MD5)Approved for entry into archive by Albirene Aires (albireneufpa@gmail.com) on 2014-09-15T12:51:43Z (GMT) No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Artigo_JcPolyomavirusInfection.pdf: 341246 bytes, checksum: 9154f8afe5e766dbe7f3364adf29e89d (MD5)Made available in DSpace on 2014-09-15T12:51:43Z (GMT). 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