Identification of fungi microflora in the ear conducts of rhesus macaques (Macaca mulatta) kept in captivity: uma abordagem estereológica

Several predisposing factors could lead to auricular diseases caused by a saprophytic microflora. Identification of the microflora of fungi could help in the diagnosis and treatment of mycoses that can become pathogenic in case of homeostasic unbalance. This report aimed to identify the saprophytic...

Full description

Bibliographic Details
Authors: Brotto, Thais Lins, Andrade, Márcia Cristina Ribeiro, Gonçalves, Miguel Ângelo Brück, Gimenis, Flávio, Pina, Alexandre
Format: article
Status:Published version
Publication Date:2005
Country:Brasil
Institution:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
Repository:Brazilian Journal of Veterinary Research and Animal Science
Language:English
OAI Identifier:oai:revistas.usp.br:article/26405
Online Access:https://www.revistas.usp.br/bjvras/article/view/26405
Access Level:Open access
Keyword:Microbiota fúngica
Primatas não-humanos
Macaco rhesus
Conduto auditivo
Fungi microflora
Non-human primates
Rhesus monkey
Ear conduct
Description
Summary:Several predisposing factors could lead to auricular diseases caused by a saprophytic microflora. Identification of the microflora of fungi could help in the diagnosis and treatment of mycoses that can become pathogenic in case of homeostasic unbalance. This report aimed to identify the saprophytic fungi microflora in the middle ear conduct of clinically healthy rhesus monkeys used for biomedical research. Forty rhesus macaques were divided into two groups. Group I was formed by adult animals, housed in individual cages inside special experimentation containers with controlled temperature and humidity. Group II, originated from the colony, was formed by young animals, which were maintained in the natural environment, without temperature and humidity control. Cerumen of the middle ear conduct of the animals was collected through swabs. Cultivation of the samples was performed in Petri plates with Sabouraud agar with cloramphenicol 1%, sealed with adhesive tape and incubated at room temperature. In the 20 animals from group I, we found the following: Aspergillus (80%), Candida (60%), Cladosporium (5%) and Rhodotorula (5%). Group II presented a major diversity of fungi: Candida sp. (95%), Aspergillus (20%), Cladosporium sp. (60%), Penicillium sp. (30%), Rodotorulla sp. (15%), Trychophytum verrucosum (5%), Epidermophyton flocosum (5%), and Scopulariopsis sp. (5%). These data will be useful for diagnoses and treatments of otites and suggest that climatic factors could be responsibles for the great number of fungi present in the animals from group II, which were exposed to natural climatic conditions.