Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents
Plant biodiversity is a source of potential natural products for the treatment of many diseases. One of the ways of discovering new drugs is through the cytotoxic screening of extract libraries. The present study evaluated 196 extracts prepared by maceration of Brazilian Atlantic Forest trees with o...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/201817 |
| Acceso en línea: | http://dx.doi.org/10.1111/iep.12351 http://hdl.handle.net/11449/201817 |
| Access Level: | acceso abierto |
| Palabra clave: | antimetastatic potential Athenaea velutina Brazilian Atlantic Forest cytotoxic activity phenolic compounds plant extract screening |
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Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agentsantimetastatic potentialAthenaea velutinaBrazilian Atlantic Forestcytotoxic activityphenolic compoundsplant extract screeningPlant biodiversity is a source of potential natural products for the treatment of many diseases. One of the ways of discovering new drugs is through the cytotoxic screening of extract libraries. The present study evaluated 196 extracts prepared by maceration of Brazilian Atlantic Forest trees with organic solvents and distilled water for cytotoxic and antimetastatic activity. The MTT assay was used to screen the extract activity in MCF-7, HepG2 and B16F10 cancer cells. The highest cytotoxic extract had antimetastatic activity, as determined in in vitro assays and melanoma murine model. The organic extract of the leaves of Athenaea velutina (EAv) significantly inhibited migration, adhesion, invasion and cell colony formation in B16F10 cells. The phenolic compounds and flavonoids in EAv were identified for the first time, using flow injection with electrospray negative ionization-ion trap tandem mass spectrometry analysis (FIA-ESI-IT-MSn). EAv markedly suppressed the development of pulmonary melanomas following the intravenous injection of melanoma cells to C57BL/6 mice. Stereological analysis of the spleen cross-sections showed enlargement of the red pulp area after EAv treatment, which indicated the activation of the haematopoietic system. The treatment of melanoma-bearing mice with EAv did not result in liver damage. In conclusion, these findings suggest that A velutina is a source of natural products with potent antimetastatic activity.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Bioquímica e Biologia Molecular Universidade Federal de ViçosaDepartamento de Biologia Geral Universidade Federal de ViçosaInstituto Federal de Educação Ciência e Tecnologia do AmazonasUNESP-Universidade Estadual PaulistaUNESP-Universidade Estadual PaulistaFAPEMIG: CDS - APQ-02540-15CNPq: GD 142313/2015-7Universidade Federal de Viçosa (UFV)Ciência e Tecnologia do AmazonasUniversidade Estadual Paulista (Unesp)2020-12-12T02:42:35Z2020-12-12T02:42:35Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article106-121http://dx.doi.org/10.1111/iep.12351International Journal of Experimental Pathology, v. 101, n. 3-4, p. 106-121, 2020.1365-26130959-9673http://hdl.handle.net/11449/20181710.1111/iep.123512-s2.0-85085568287Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Experimental Pathologyinfo:eu-repo/semantics/openAccessAlmeida, Alisson A.Lima, Graziela D. A.Simão, Marcos V. R. C.Moreira, Gabriela A.Siqueira, Raoni P.Zanatta, Ana C. [UNESP]Vilegas, Wagner [UNESP]Machado-Neves, MarianaBressan, Gustavo C.Leite, João P. V.2024-10-24T12:55:14Zoai:repositorio.unesp.br:11449/201817Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-24T12:55:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| title |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| spellingShingle |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents Almeida, Alisson A. antimetastatic potential Athenaea velutina Brazilian Atlantic Forest cytotoxic activity phenolic compounds plant extract screening |
| title_short |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| title_full |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| title_fullStr |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| title_full_unstemmed |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| title_sort |
Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents |
| dc.creator.none.fl_str_mv |
Almeida, Alisson A. Lima, Graziela D. A. Simão, Marcos V. R. C. Moreira, Gabriela A. Siqueira, Raoni P. Zanatta, Ana C. [UNESP] Vilegas, Wagner [UNESP] Machado-Neves, Mariana Bressan, Gustavo C. Leite, João P. V. |
| author |
Almeida, Alisson A. |
| author_facet |
Almeida, Alisson A. Lima, Graziela D. A. Simão, Marcos V. R. C. Moreira, Gabriela A. Siqueira, Raoni P. Zanatta, Ana C. [UNESP] Vilegas, Wagner [UNESP] Machado-Neves, Mariana Bressan, Gustavo C. Leite, João P. V. |
| author_role |
author |
| author2 |
Lima, Graziela D. A. Simão, Marcos V. R. C. Moreira, Gabriela A. Siqueira, Raoni P. Zanatta, Ana C. [UNESP] Vilegas, Wagner [UNESP] Machado-Neves, Mariana Bressan, Gustavo C. Leite, João P. V. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Ciência e Tecnologia do Amazonas Universidade Estadual Paulista (Unesp) |
| dc.subject.por.fl_str_mv |
antimetastatic potential Athenaea velutina Brazilian Atlantic Forest cytotoxic activity phenolic compounds plant extract screening |
| topic |
antimetastatic potential Athenaea velutina Brazilian Atlantic Forest cytotoxic activity phenolic compounds plant extract screening |
| description |
Plant biodiversity is a source of potential natural products for the treatment of many diseases. One of the ways of discovering new drugs is through the cytotoxic screening of extract libraries. The present study evaluated 196 extracts prepared by maceration of Brazilian Atlantic Forest trees with organic solvents and distilled water for cytotoxic and antimetastatic activity. The MTT assay was used to screen the extract activity in MCF-7, HepG2 and B16F10 cancer cells. The highest cytotoxic extract had antimetastatic activity, as determined in in vitro assays and melanoma murine model. The organic extract of the leaves of Athenaea velutina (EAv) significantly inhibited migration, adhesion, invasion and cell colony formation in B16F10 cells. The phenolic compounds and flavonoids in EAv were identified for the first time, using flow injection with electrospray negative ionization-ion trap tandem mass spectrometry analysis (FIA-ESI-IT-MSn). EAv markedly suppressed the development of pulmonary melanomas following the intravenous injection of melanoma cells to C57BL/6 mice. Stereological analysis of the spleen cross-sections showed enlargement of the red pulp area after EAv treatment, which indicated the activation of the haematopoietic system. The treatment of melanoma-bearing mice with EAv did not result in liver damage. In conclusion, these findings suggest that A velutina is a source of natural products with potent antimetastatic activity. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-12T02:42:35Z 2020-12-12T02:42:35Z 2020-06-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iep.12351 International Journal of Experimental Pathology, v. 101, n. 3-4, p. 106-121, 2020. 1365-2613 0959-9673 http://hdl.handle.net/11449/201817 10.1111/iep.12351 2-s2.0-85085568287 |
| url |
http://dx.doi.org/10.1111/iep.12351 http://hdl.handle.net/11449/201817 |
| identifier_str_mv |
International Journal of Experimental Pathology, v. 101, n. 3-4, p. 106-121, 2020. 1365-2613 0959-9673 10.1111/iep.12351 2-s2.0-85085568287 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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International Journal of Experimental Pathology |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
106-121 |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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1853672215706337280 |
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15.301603 |