Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations

The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconameric...

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Autores: Santos, Angelica Rossotti dos, Usso, Mariana Campaner, Gouveia, Juceli Gonzalez, Araya-Jaime, Cristian [UNESP], Frantine-Silva, Wilson, Giuliano-Caetano, Lucia, Foresti, Fausto [UNESP], Dias, Ana Lucia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/162854
Acceso en línea:http://dx.doi.org/10.1089/zeb.2016.1380
http://hdl.handle.net/11449/162854
Access Level:acceso abierto
Palabra clave:fish cytogenetics
molecular cytogenetics
ribosomal DNA
U2 snDNA
karyotypic polymorphism
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spelling Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populationsfish cytogeneticsmolecular cytogeneticsribosomal DNAU2 snDNAkaryotypic polymorphismThe mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter-and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Londrina, Dept Biol Geral, Lab Citogenet Anim, Rodovia Celso Garcia Cida, BR-86057970 Londrina, BrazilUniv Chile, ICBM Fac Med, Lab Citogenet Vertebrados, Santiago, ChileUniv Estadual Londrina, Dept Biol Geral, Lab Genet & Ecol Anim, Londrina, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilMary Ann Liebert, IncUniversidade Estadual de Londrina (UEL)Univ ChileUniversidade Estadual Paulista (Unesp)2018-11-26T17:34:41Z2018-11-26T17:34:41Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article261-271http://dx.doi.org/10.1089/zeb.2016.1380Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017.1545-8547http://hdl.handle.net/11449/16285410.1089/zeb.2016.1380WOS:000402566000008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZebrafish0,795info:eu-repo/semantics/openAccessSantos, Angelica Rossotti dosUsso, Mariana CampanerGouveia, Juceli GonzalezAraya-Jaime, Cristian [UNESP]Frantine-Silva, WilsonGiuliano-Caetano, LuciaForesti, Fausto [UNESP]Dias, Ana Lucia2025-10-23T09:51:03Zoai:repositorio.unesp.br:11449/162854Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-23T09:51:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
title Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
spellingShingle Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
Santos, Angelica Rossotti dos
fish cytogenetics
molecular cytogenetics
ribosomal DNA
U2 snDNA
karyotypic polymorphism
title_short Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
title_full Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
title_fullStr Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
title_full_unstemmed Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
title_sort Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
dc.creator.none.fl_str_mv Santos, Angelica Rossotti dos
Usso, Mariana Campaner
Gouveia, Juceli Gonzalez
Araya-Jaime, Cristian [UNESP]
Frantine-Silva, Wilson
Giuliano-Caetano, Lucia
Foresti, Fausto [UNESP]
Dias, Ana Lucia
author Santos, Angelica Rossotti dos
author_facet Santos, Angelica Rossotti dos
Usso, Mariana Campaner
Gouveia, Juceli Gonzalez
Araya-Jaime, Cristian [UNESP]
Frantine-Silva, Wilson
Giuliano-Caetano, Lucia
Foresti, Fausto [UNESP]
Dias, Ana Lucia
author_role author
author2 Usso, Mariana Campaner
Gouveia, Juceli Gonzalez
Araya-Jaime, Cristian [UNESP]
Frantine-Silva, Wilson
Giuliano-Caetano, Lucia
Foresti, Fausto [UNESP]
Dias, Ana Lucia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Univ Chile
Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv fish cytogenetics
molecular cytogenetics
ribosomal DNA
U2 snDNA
karyotypic polymorphism
topic fish cytogenetics
molecular cytogenetics
ribosomal DNA
U2 snDNA
karyotypic polymorphism
description The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter-and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2018-11-26T17:34:41Z
2018-11-26T17:34:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1089/zeb.2016.1380
Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017.
1545-8547
http://hdl.handle.net/11449/162854
10.1089/zeb.2016.1380
WOS:000402566000008
url http://dx.doi.org/10.1089/zeb.2016.1380
http://hdl.handle.net/11449/162854
identifier_str_mv Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017.
1545-8547
10.1089/zeb.2016.1380
WOS:000402566000008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zebrafish
0,795
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 261-271
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc
publisher.none.fl_str_mv Mary Ann Liebert, Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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