Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection

The data presented in this study are available on request from the corresponding authors. The obtained nucleotide sequences were deposited in GenBank under the following accession numbers OR179883, OR179882, OR179881, OR179884, OR179887, OR179885, OR179889, OR179888 and OR179886.

Detalles Bibliográficos
Autores: Arruda, Thaís Bonato, Bavia, Lorena, Mosimann, Ana Luiza Pamplona, Aoki, Mateus Nóbrega, Sarzi, Maria Lo, Conchon-Costa, Ivete, Wowk, Pryscilla Fanini, Santos, Claudia Nunes Duarte, Pavanelli, Wander Rogério, Silveira, Guilherme Ferreira, Bordignon, Juliano
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/67525
Acceso en línea:https://arca.fiocruz.br/handle/icict/67525
Access Level:acceso abierto
Palabra clave:Dengue virus
Fase aguda da dengue
Citocinas
IL-2
Viremia
Dengue Virus
Dengue acute phase
Cytokines
Vírus da Dengue
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dc.title.none.fl_str_mv Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
title Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
spellingShingle Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
Arruda, Thaís Bonato
Dengue virus
Fase aguda da dengue
Citocinas
IL-2
Viremia
Dengue Virus
Dengue acute phase
Cytokines
IL-2
Viremia
Vírus da Dengue
Citocinas
Viremia
title_short Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
title_full Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
title_fullStr Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
title_full_unstemmed Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
title_sort Viremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infection
dc.creator.none.fl_str_mv Arruda, Thaís Bonato
Bavia, Lorena
Mosimann, Ana Luiza Pamplona
Aoki, Mateus Nóbrega
Sarzi, Maria Lo
Conchon-Costa, Ivete
Wowk, Pryscilla Fanini
Santos, Claudia Nunes Duarte
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Bordignon, Juliano
author Arruda, Thaís Bonato
author_facet Arruda, Thaís Bonato
Bavia, Lorena
Mosimann, Ana Luiza Pamplona
Aoki, Mateus Nóbrega
Sarzi, Maria Lo
Conchon-Costa, Ivete
Wowk, Pryscilla Fanini
Santos, Claudia Nunes Duarte
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Bordignon, Juliano
author_role author
author2 Bavia, Lorena
Mosimann, Ana Luiza Pamplona
Aoki, Mateus Nóbrega
Sarzi, Maria Lo
Conchon-Costa, Ivete
Wowk, Pryscilla Fanini
Santos, Claudia Nunes Duarte
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Bordignon, Juliano
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Dengue virus
Fase aguda da dengue
Citocinas
IL-2
Viremia
topic Dengue virus
Fase aguda da dengue
Citocinas
IL-2
Viremia
Dengue Virus
Dengue acute phase
Cytokines
IL-2
Viremia
Vírus da Dengue
Citocinas
Viremia
dc.subject.en.none.fl_str_mv Dengue Virus
Dengue acute phase
Cytokines
IL-2
Viremia
dc.subject.decs.none.fl_str_mv Vírus da Dengue
Citocinas
Viremia
description The data presented in this study are available on request from the corresponding authors. The obtained nucleotide sequences were deposited in GenBank under the following accession numbers OR179883, OR179882, OR179881, OR179884, OR179887, OR179885, OR179889, OR179888 and OR179886.
publishDate 2023
dc.date.issued.fl_str_mv 2023
dc.date.accessioned.fl_str_mv 2024-12-11T00:28:58Z
dc.date.available.fl_str_mv 2024-12-11T00:28:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ARRUDA, T. B. et al. Viremia and inflammatory cytokines in dengue: interleukin -2 as a biomarker of infection, and interferon-α and -γ as markers of primary versus secondary infection. Pathogens, v. 12, n. 1362, p. 1-18, 2023.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/67525
dc.identifier.issn.none.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens12111362
identifier_str_mv ARRUDA, T. B. et al. Viremia and inflammatory cytokines in dengue: interleukin -2 as a biomarker of infection, and interferon-α and -γ as markers of primary versus secondary infection. Pathogens, v. 12, n. 1362, p. 1-18, 2023.
2076-0817
10.3390/pathogens12111362
url https://arca.fiocruz.br/handle/icict/67525
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2024-12-11T00:28:58Z2024-12-11T00:28:58Z2023ARRUDA, T. B. et al. Viremia and inflammatory cytokines in dengue: interleukin -2 as a biomarker of infection, and interferon-α and -γ as markers of primary versus secondary infection. Pathogens, v. 12, n. 1362, p. 1-18, 2023.2076-0817https://arca.fiocruz.br/handle/icict/6752510.3390/pathogens12111362The data presented in this study are available on request from the corresponding authors. The obtained nucleotide sequences were deposited in GenBank under the following accession numbers OR179883, OR179882, OR179881, OR179884, OR179887, OR179885, OR179889, OR179888 and OR179886.This research was funded by the Brazilian Ministry of Health and Fundação Araucária/SESA-PR/CNPq/MS-Decit PPSUS/2015, 009/2017, 019/2017, 041/2017. C.N.D.d.S. (307176/2018-5), W.R.P. (301594/2018-0 and 305059/2021-1), and J.B. (312671/2020-2 and 205096/2018-2) are CNPq fellowsFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil / Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Secretaria Municipal de Saúde de Cambé. Cambé, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Protozoologia Experimental. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Protozoologia Experimental. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.The pathogenesis of Dengue virus (DENV) infection is complex and involves viral replication that may trigger an inflammatory response leading to severe disease. Here, we investigated the correlation between viremia and cytokine levels in the serum of DENV-infected patients. Between 2013 and 2014, 138 patients with a diagnosis of acute-phase DENV infection and 22 patients with a non-dengue acute febrile illness (AFI) were enrolled. Through a focus-forming assay (FFU), we determined the viremia levels in DENV-infected patients and observed a peak in the first two days after the onset of symptoms. A higher level of viremia was observed in primary versus secondary DENV-infected patients. Furthermore, no correlation was observed between viremia and inflammatory cytokine levels in DENV-infected patients. Receiver operating characteristic (ROC) curve analysis revealed that IL-2 has the potential to act as a marker to distinguish dengue from other febrile illnesses and is positively correlated with Th1 cytokines. IFN-α and IFN-γ appear to be potential markers of primary versus secondary infection in DENV-infected patients, respectively. The results also indicate that viremia levels are not the main driving force behind inflammation in dengue and that cytokines could be used as infection biomarkers and for differentiation between primary versus secondary infection.engMDPIDengue virusFase aguda da dengueCitocinasIL-2ViremiaDengue VirusDengue acute phaseCytokinesIL-2ViremiaVírus da DengueCitocinasViremiaViremia and inflammatory cytokines in dengue: interleukin-2 as a biomarker of infection, and Interferon-α and -γ as markers of primary versus secondary infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZArruda, Thaís BonatoBavia, LorenaMosimann, Ana Luiza PamplonaAoki, Mateus NóbregaSarzi, Maria LoConchon-Costa, IveteWowk, Pryscilla FaniniSantos, Claudia Nunes DuartePavanelli, Wander RogérioSilveira, Guilherme FerreiraBordignon, JulianoLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/1bbc709b-7eda-4f73-8941-2aded28a9354/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALve_Thaís_Arruda_etal_ICC_2023.pdfve_Thaís_Arruda_etal_ICC_2023.pdfapplication/pdf2404668https://arca.fiocruz.br/bitstreams/b56beb85-bc42-414c-ba8f-4fee1567d328/downloade6795a74f1b6dc571526923b31548ffeMD53trueAnonymousREADTEXTve_Thaís_Arruda_etal_ICC_2023.pdf.txtve_Thaís_Arruda_etal_ICC_2023.pdf.txtExtracted texttext/plain76669https://arca.fiocruz.br/bitstreams/0fa416bc-336b-49fd-8b0f-1bdda3da9634/download8e0b5d6e013458504be2217b9dbddd3aMD512falseAnonymousREADTHUMBNAILve_Thaís_Arruda_etal_ICC_2023.pdf.jpgve_Thaís_Arruda_etal_ICC_2023.pdf.jpgGenerated Thumbnailimage/jpeg43511https://arca.fiocruz.br/bitstreams/6f07071a-9127-4c51-be5e-cb2ce407ee27/download97852d3b9d57e42f32056d8de0b92be5MD513falseAnonymousREADicict/675252025-12-10 13:48:47.021open.accessoai:arca.fiocruz.br:icict/67525https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T16:48:47Repositório Institucional da FIOCRUZ (ARCA) - 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