Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain

Many studies indicate that thimet oligopeptidase (EC3.4.24.15; TOP) can be implicated in the metabolism of bioactive peptides, including dynorphin 1-8, alpha-neoendorphin, beta-neoendorphin and GnRH. Furthermore, the higher levels of this peptidase are found in neuroendocrine tissue and testis. in t...

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Detalles Bibliográficos
Autores: Dalio, Fernanda Montiel [UNIFESP], Visniauskas, Bruna [UNIFESP], Bicocchi, Eliane S., Perry, Juliana Cini [UNIFESP], Freua, Rodrigo [UNIFESP], Gesteira, Tarsis Ferreira [UNIFESP], Nader, Helena Bonciani [UNIFESP], Machado, Mauricio Ferreira Marcondes [UNIFESP], Tufik, Sergio [UNIFESP], Ferro, Emer Suavinho [UNIFESP], Andersen, Monica Levy [UNIFESP], Toledo, Claudio A. B., Chagas, Jair Ribeiro [UNIFESP], Oliveira, Vitor [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/34717
Acceso en línea:http://dx.doi.org/10.1016/j.bbrc.2012.02.088
http://repositorio.unifesp.br/handle/11600/34717
Access Level:acceso abierto
Palabra clave:Cocaine
EC3.4.24.15
Neuropeptidase
Striatum
Thimet oligopeptidase
Descripción
Sumario:Many studies indicate that thimet oligopeptidase (EC3.4.24.15; TOP) can be implicated in the metabolism of bioactive peptides, including dynorphin 1-8, alpha-neoendorphin, beta-neoendorphin and GnRH. Furthermore, the higher levels of this peptidase are found in neuroendocrine tissue and testis. in the present study, we have evaluated the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry; ventral striatum, hippocampus, and frontal cortex. No significant differences on TOP specific activity were detected in the hippocampus and frontal cortex of cocaine treated animals compared to control vehicle group. However, a significant increase in activity was observed in the ventral striatum of cocaine treated-rats. the increase occurred in both, TOP specific activity and TOP relative mRNA amount determined by real time RT-PCR. As TOP can be implicated in the processing of many neuropeptides, and previous studies have shown that cocaine also alters the gene expression of proenkephalin and prodynorphin in the striatum, the present findings suggest that TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. (C) 2012 Elsevier Inc. All rights reserved.

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