Comparison between immunoassay and thin-layer chromatography for determination of aflatoxins, ochratoxin A and zearalenone in corn and com meal
Twotechniqucs, one ofthem using thin-layer chromatography and the other enzyme-Iinked irrununosorbent assay (ELISA) were compared for the detennination of aflatoxins, ochratoxin A and zearalenone. Seven sampIes of com and twenty samp1csof com meal from an industry in Paraná State were analysed for t...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1998 |
| País: | Brasil |
| Institución: | Instituto Adolfo Lutz |
| Repositorio: | Revista do Instituto Adolfo Lutz (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/36623 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/RIAL/article/view/36623 |
| Access Level: | acceso abierto |
| Palabra clave: | ELISA thin-layer chromatography imunoassay aflatoxins ochratoxin A zearalenone corn and corn meal cromatografia em camada delgada imunoensaio aflatoxinas ocratoxina A zearalenona milho fubá |
| Sumario: | Twotechniqucs, one ofthem using thin-layer chromatography and the other enzyme-Iinked irrununosorbent assay (ELISA) were compared for the detennination of aflatoxins, ochratoxin A and zearalenone. Seven sampIes of com and twenty samp1csof com meal from an industry in Paraná State were analysed for this purpose.The ELISA method was simpler to use, faster, showing higher concentrations than thc thin-layer chromatography mcthod. False positives requiring confirmation of the mycotoxins were observed with the ELISA method. |
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